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PAX7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248402

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and… More details

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Product Overview

Online Datasheet in PDF
Product Name
PAX7, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PAX7
Product Gene Name
anti-PAX7 antibody
Product Synonym Names
Anti-PAX7 Antibody (aa410-437) IHC-plus; PAX7; HUP1; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; RMS2; Paired domain gene 7; PAX7 transcriptional factor; PAX7B; Human PAX7
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Specificity
Human PAX7
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
OMIM
gene 268220
3D Structure
ModBase 3D Structure for P23759
Target Species
Human
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human PAX7.
Immunogen Type
Synthetic peptide
Immunogen
PAX7 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human PAX7.
Epitope
aa410-437
Specificity Note
This PAX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-437 amino acids from the C-terminal region of human PAX7.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-PAX7 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-PAX7 antibody
IHC-P (5 ug/ml), WB (1:1000)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-PAX7 antibody

Anti-PAX7 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-PAX7 Polyclonal Antibody – Cat# MBS248402

Western Blot (WB) of anti-PAX7 antibody

PAX7 Antibody (C-term) Western blot of mouse spleen tissue lysates (35 ug/lane). This demonstrates the PAX7 antibody detected the PAX7 protein (arrow).
Rabbit Anti-PAX7 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248402

Other Notes

Small volumes of anti-PAX7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAX7 purchase

MBS248402 is an antibody targeting PAX7. Also known as Rabbit Polyclonal to Human PAX7. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAX7. It may not necessarily be applicable to this product.

NCBI GI #
4505619
NCBI GeneID
5081
NCBI Accession #
NP_002575.1
UniProt Primary Accession #
P23759
UniProt Secondary Accession #
Q0VA99; Q2PJS5; E9PFV9
UniProt Related Accession #
P23759
GenBank Nucleotide #
NM_002584.2
NCBI Official Full Name
paired box protein Pax-7 isoform 1
NCBI Official Synonym Full Names
paired box 7
NCBI Official Symbol
PAX7
NCBI Official Synonym Symbols
HUP1; RMS2; PAX7B
UniProt Protein Name
Paired box protein Pax-7
UniProt Synonym Protein Names
HuP1
Protein Family
Paired box protein
UniProt Gene Name
PAX7
UniProt Synonym Gene Names
HUP1
UniProt Entry Name
PAX7_HUMAN
NCBI Protein Information
paired box protein Pax-7; paired domain gene 7; paired box homeotic gene 7; PAX7 transcriptional factor

NCBI Summary for PAX7

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
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UniProt Comments for PAX7

PAX7: Probable transcription factor. May have a role in myogenesis. Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2). It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; positive regulation of histone methylation; embryonic skeletal development; satellite cell commitment; neuron fate commitment; skeletal muscle regeneration; chromatin remodeling; regulation of protein binding; cartilage development; regulation of cell fate commitment; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; dorsoventral neural tube patterning; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2
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Research Articles on PAX7

1. RAGE upregulates myogenin which upregulates MyoD and downregulates PAX7, with consequent inhibition of proliferation and stimulation of differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAX7 antibody

 Reference Product  PubMed Publications
 MYF5 antibody  >120 publications with PAX7 and MYF5
 MYOD1 antibody  >86 publications with PAX7 and MYOD1
 MYOG antibody  >41 publications with PAX7 and MYOG
 SOX10 antibody  >14 publications with PAX7 and SOX10
 MSTN antibody  >10 publications with PAX7 and MSTN
 WDR5 antibody  >4 publications with PAX7 and WDR5
 ASH2L antibody  >3 publications with PAX7 and ASH2L

Pathways associated with anti-PAX7 antibody

 Products by Pathway  Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 MiRs In Muscle Cell Differentiation Pathway antibodies  MiRs In Muscle Cell Differentiation Pathway Diagram

Diseases associated with anti-PAX7 antibody

 Disease Name  Pubmed Publications
 Rhabdomyosarcoma Antibodies  >159 publications with PAX7 and Rhabdomyosarcoma
 Rhabdomyosarcoma, Alveolar Antibodies  >133 publications with PAX7 and Rhabdomyosarcoma, Alveolar
 Nervous System Diseases Antibodies  >109 publications with PAX7 and Nervous System Diseases
 Disease Models, Animal Antibodies  >49 publications with PAX7 and Disease Models, Animal
 Necrosis Antibodies  >29 publications with PAX7 and Necrosis
 Fibrosis Antibodies  >27 publications with PAX7 and Fibrosis
 Hyperplasia Antibodies  >17 publications with PAX7 and Hyperplasia
 Skin Diseases Antibodies  >12 publications with PAX7 and Skin Diseases
 Weight Loss Antibodies  >9 publications with PAX7 and Weight Loss
 Neoplasm Metastasis Antibodies  >5 publications with PAX7 and Neoplasm Metastasis

Organs/Tissues associated with anti-PAX7 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >180 publications with PAX7 and Embryonic Tissue
 Brain Antibodies  >69 publications with PAX7 and Brain
 Eye Antibodies  >45 publications with PAX7 and Eye
 Connective Tissue Antibodies  >20 publications with PAX7 and Connective Tissue
 Kidney Antibodies  >4 publications with PAX7 and Kidney
 Testis Antibodies  >2 publications with PAX7 and Testis
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