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SPATA18 Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248459

MBS248459 is an antibody targeting SPATA18. Also known as Chicken Polyclonal (IgY) to Human SPATA18 / MIEAP. Reactive in Human, Mouse, Rat. Host: Chicken. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SPATA18 / MIEAP, Polyclonal Antibody
Full Product Name
Chicken Polyclonal (IgY) to Human SPATA18 / MIEAP
Product Gene Name
anti-SPATA18 antibody
Product Synonym Gene Name
MIEAP
Product Synonym Names
Anti-SPATA18 / MIEAP Antibody (N-Terminus) IHC-plus; SPATA18; Mieap; SPETEX1; Spermatogenesis associated 18; Mitochondria-eating protein; Human SPATA18; MIEAP
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SPATA18. At least three isoforms of SPATA18 are known to exist; this antibody will detect the two longest isoforms. SPATA18 antibody is predicted to not cross-react with other SPATA family members.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
57,485 Da
OMIM
612814
3D Structure
ModBase 3D Structure for Q8TC71
Target Species
Human
Immunogen Description
A 19 amino acid peptide near the amino terminus of human SPATA18
Immunogen Type
Synthetic peptide
Immunogen
SPATA18 / MIEAP antibody was raised against a 19 amino acid peptide near the amino terminus of human SPATA18
Antigen Modification
N-Terminus
Applications Tested/Suitable for anti-SPATA18 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SPATA18 antibody
ELISA, IHC-P (5 ug/ml), WB (1 ug/ml)

Immunohistochemistry (IHC) of anti-SPATA18 antibody

Anti-SPATA18 / MIEAP antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Chicken Anti-SPATA18 Polyclonal Antibody – Cat# MBS248459

Western Blot (WB) of anti-SPATA18 antibody

Western blot analysis of SPATA18 in rat lung tissue lysate with SPATA18 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide
Chicken Anti-SPATA18 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248459

Other Notes

Small volumes of anti-SPATA18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPATA18. It may not necessarily be applicable to this product.

NCBI GI #
21687119
NCBI GeneID
132671
NCBI Accession #
NP_660306.1
UniProt Primary Accession #
Q8TC71
UniProt Secondary Accession #
Q8IY48; Q8N7D7; B4E2R0; E5RLK1
UniProt Related Accession #
Q8TC71
GenBank Nucleotide #
NM_145263.3
NCBI Official Full Name
mitochondria-eating protein isoform 1
NCBI Official Synonym Full Names
spermatogenesis associated 18
NCBI Official Symbol
SPATA18
NCBI Official Synonym Symbols
Mieap; SPETEX1
UniProt Protein Name
Mitochondria-eating protein
UniProt Synonym Protein Names
Spermatogenesis-associated protein 18
Protein Family
Mitochondria-eating protein
UniProt Gene Name
SPATA18
UniProt Synonym Gene Names
MIEAP
UniProt Entry Name
MIEAP_HUMAN
NCBI Protein Information
mitochondria-eating protein; spermatogenesis associated 18 homolog; spermatogenesis-associated protein 18

UniProt Comments for SPATA18

SPATA18: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Belongs to the MIEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: protein binding

Biological Process: response to DNA damage stimulus
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Research Articles on SPATA18

1. The physical interaction of Mieap, BNIP3 and NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPATA18 antibody

 Reference Product  PubMed Publications
 SGCB antibody  >1 publications with SPATA18 and SGCB

Diseases associated with anti-SPATA18 antibody

 Disease Name  Pubmed Publications
 Urinary Bladder Neoplasms Antibodies  >1 publications with SPATA18 and Urinary Bladder Neoplasms

Organs/Tissues associated with anti-SPATA18 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >3 publications with SPATA18 and Testis
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