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NFIA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248474

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
NFIA / Nuclear Factor 1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human NFIA / Nuclear Factor 1
Product Gene Name
anti-NFIA antibody
Product Synonym Names
Anti-NFIA / Nuclear Factor 1 Antibody (aa469-498) IHC-plus; NFIA; CTF; NF1-A; NF-I/A; NFI-L; Nuclear Factor 1; TGGCA-binding protein; NFI-A; Nuclear factor 1 A-type; KIAA1439; Nuclear factor 1/A; Nuclear factor I/A; Human NFIA; Nuclear Factor 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Specificity
Human NFIA / Nuclear Factor 1
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
60,894 Da
OMIM
600727
3D Structure
ModBase 3D Structure for Q12857
Target Species
Human
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human NFIA.
Immunogen Type
Synthetic peptide
Immunogen
NFIA / Nuclear Factor 1 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human NFIA.
Antigen Modification
aa469-498
Applications Tested/Suitable for anti-NFIA antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-NFIA antibody
IF (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000)

Immunohistochemistry (IHC) of anti-NFIA antibody

Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-NFIA Polyclonal Antibody – Cat# MBS248474

Western Blot (WB) of anti-NFIA antibody

NFIA Antibody (C-term) Western blot of HepG2 cell line lysates (35 ug/lane). This demonstrates the NFIA antibody detected the NFIA protein (arrow).
Rabbit Anti-NFIA Polyclonal Antibody, Western Blot (WB) – Cat# MBS248474

Other Notes

Small volumes of anti-NFIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFIA purchase

MBS248474 is an antibody targeting NFIA. Also known as Rabbit Polyclonal to Human NFIA / Nuclear Factor 1. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NFIA. It may not necessarily be applicable to this product.

NCBI GI #
30840980
NCBI GeneID
4774
NCBI Accession #
NP_005586.1
UniProt Primary Accession #
Q12857
UniProt Secondary Accession #
Q8TA97; Q9H3X9; Q9P2A9; B4DRJ3; B4DS53; F5H0R0; F8W8W3
UniProt Related Accession #
Q12857
GenBank Nucleotide #
NM_005595.4
NCBI Official Full Name
nuclear factor 1 A-type isoform 2
NCBI Official Synonym Full Names
nuclear factor I/A
NCBI Official Symbol
NFIA
NCBI Official Synonym Symbols
CTF; NF1-A; NFI-A; NFI-L; NF-I/A
UniProt Protein Name
Nuclear factor 1 A-type
UniProt Synonym Protein Names
CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein
Protein Family
Nuclear factor
UniProt Gene Name
NFIA
UniProt Synonym Gene Names
KIAA1439; NF1-A; Nuclear factor 1/A; CTF; NF-I/A; NFI-A
UniProt Entry Name
NFIA_HUMAN
NCBI Protein Information
nuclear factor 1 A-type; TGGCA-binding protein; CCAAT-box-binding transcription factor

NCBI Summary for NFIA

This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for NFIA

NFI-A: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p31.3-p31.2

Cellular Component: nucleoplasm; cell junction; nucleus

Molecular Function: transcription factor binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; DNA replication; response to organic cyclic substance
Show more

Research Articles on NFIA

1. High nuclear factor IA expression is associated with glioblastomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NFIA antibody

 Products by Pathway  Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Termination Pathway antibodies  RNA Polymerase III Transcription Termination Pathway Diagram

Diseases associated with anti-NFIA antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >17 publications with NFIA and Nervous System Diseases
 Congenital Abnormalities Antibodies  >7 publications with NFIA and Congenital Abnormalities
 Urogenital Abnormalities Antibodies  >2 publications with NFIA and Urogenital Abnormalities
 Inflammation Antibodies  >2 publications with NFIA and Inflammation
 Disorders of Sex Development Antibodies  >2 publications with NFIA and Disorders of Sex Development
 Kidney Diseases Antibodies  >2 publications with NFIA and Kidney Diseases
 Craniofacial Abnormalities Antibodies  >1 publications with NFIA and Craniofacial Abnormalities
 Disease Models, Animal Antibodies  >1 publications with NFIA and Disease Models, Animal
 Learning Disorders Antibodies  >1 publications with NFIA and Learning Disorders
 Hypertrophy Antibodies  >1 publications with NFIA and Hypertrophy

Organs/Tissues associated with anti-NFIA antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >40 publications with NFIA and Brain
 Embryonic Tissue Antibodies  >12 publications with NFIA and Embryonic Tissue
 Lung Antibodies  >8 publications with NFIA and Lung
 Thyroid Antibodies  >7 publications with NFIA and Thyroid
 Bone Antibodies  >7 publications with NFIA and Bone
 Liver Antibodies  >6 publications with NFIA and Liver
 Kidney Antibodies  >5 publications with NFIA and Kidney
 Eye Antibodies  >4 publications with NFIA and Eye
 Muscle Antibodies  >3 publications with NFIA and Muscle
 Heart Antibodies  >3 publications with NFIA and Heart
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