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GLO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248477

MBS248477 is an antibody targeting GLO1. Also known as Rabbit Polyclonal (IgG) to Human GLO1 / Glyoxalase I. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Overview

Product Name
GLO1 / Glyoxalase I, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human GLO1 / Glyoxalase I
Product Gene Name
Product Synonym Names
Anti-GLO1 / Glyoxalase I Antibody (aa1-184) IHC-plus; GLO1; Aldoketomutase; Glx I; Glyoxalase domain containing 1; Glyoxalase I; Lactoyl glutathione lyase; Lactoylglutathione lyase; GLOD1; Methylglyoxalase; Ketone-aldehyde mutase; GLYI; Human GLO1; Glyoxalase I
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human GLO1 / Glyoxalase I
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.
Molecular Weight
19,043 Da
OMIM
138750
3D Structure
ModBase 3D Structure for Q04760
Target Species
Human
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 1 and 184 of Glyoxalase I (SwissProt Q04760). Percent identity by BLAST analysis: Human (100%); Rat (91%); Bovine (90%); Xenopus (82%).
Immunogen Type
Recombinant protein
Immunogen
GLO1 / Glyoxalase I antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 184 of Glyoxalase I (SwissProt Q04760). Percent identity by BLAST analysis: Human (100%); Rat (91%); Bovine (90%); Xenopus (82%).
Antigen Modification
aa1-184
Applications Tested/Suitable for anti-GLO1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-GLO1 antibody
ICC (1:100 - 1:1000), IHC-P (7.5 ug/ml), WB (1:500 - 1:3000)
Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Immunohistochemistry (IHC) of anti-GLO1 antibody

Anti-GLO1 / Glyoxalase I antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Rabbit Anti-GLO1 Polyclonal Antibody – Cat# MBS248477

Immunocytochemistry (ICC) of anti-GLO1 antibody

Glyoxalase I antibody [N1C3] detects GLO1 protein at cytoplasm and nucleus by immunofluorescent analysis. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. GLO1 protein stained by Glyoxalase I antibody [N1C3] diluted at 1:500.
Rabbit Anti-GLO1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS248477

Western Blot (WB) of anti-GLO1 antibody

Sample (30g whole cell lysate). A:293T, B: A431, C: H1299, D: HeLa S3, E: Hep G2. F: MOLT4. G: Raji. 12% SDS PAGE. GLO1 / Glyoxalase I antibody diluted at 1:1000
Rabbit Anti-GLO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248477

Western Blot (WB) of anti-GLO1 antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. GLO1 / Glyoxalase I antibody diluted at 1:1000.
Rabbit Anti-GLO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248477

Other Notes

Small volumes of anti-GLO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GLO1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lactoylglutathione lyase
NCBI Official Synonym Full Names
glyoxalase I
NCBI Official Symbol
GLO1
NCBI Official Synonym Symbols
GLYI; GLOD1; HEL-S-74
UniProt Protein Name
Lactoylglutathione lyase
UniProt Synonym Protein Names
Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
UniProt Gene Name
GLO1
UniProt Synonym Gene Names
Glx I
UniProt Entry Name
LGUL_HUMAN
NCBI Protein Information
lactoylglutathione lyase; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; glyoxalase domain containing 1; epididymis secretory protein Li 74; S-D-lactoylglutathione methylglyoxal lyase

NCBI Summary for GLO1

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

UniProt Comments for GLO1

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; EC 4.4.1.5; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 6p21.3-p21.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; lactoylglutathione lyase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; methylglyoxal metabolic process; negative regulation of apoptosis

Disease: Autism
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GLO1 antibody

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