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Carboxypeptidase E / CPE (CPE) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248504

Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Carboxypeptidase e / CPE, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human Carboxypeptidase e / CPE
Product Gene Name
Product Synonym Names
Anti-Carboxypeptidase e / CPE Antibody (aa271-320) IHC-plus; CPE; Carboxypeptidase E; CPH; Carboxypeptidase H; Enkephalin convertase; Human Carboxypeptidase e; CPE
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
CPE Antibody detects endogenous levels of total CPE protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
49,886 Da
OMIM
114855
3D Structure
ModBase 3D Structure for P16870
Target Species
Human
Immunogen Description
Synthetic peptide from human CPE (aa271-320).
Immunogen
Carboxypeptidase e / CPE antibody was raised against synthetic peptide from human CPE (aa271-320).
Antigen Modification
aa271-320
Applications Tested/Suitable for anti-CPE antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CPE antibody
ELISA (1:5000), IHC-P (20 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-CPE antibody

Anti-Carboxypeptidase E / CPE antibody IHC staining of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-CPE Polyclonal Antibody – Cat# MBS248504

Western Blot (WB) of anti-CPE antibody

Western blot of extracts from RAW264.7/NIH-3T3 cells, using CPE Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-CPE Polyclonal Antibody, Western Blot (WB) – Cat# MBS248504

Other Notes

Small volumes of anti-CPE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPE purchase

MBS248504 is an antibody targeting CPE. Also known as Rabbit Polyclonal (IgG) to Human Carboxypeptidase e / CPE. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CPE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carboxypeptidase E preproprotein
NCBI Official Synonym Full Names
carboxypeptidase E
NCBI Official Symbol
CPE
UniProt Protein Name
Carboxypeptidase E
UniProt Synonym Protein Names
Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase
Protein Family
UniProt Gene Name
CPE
UniProt Synonym Gene Names
CPE; CPH
UniProt Entry Name
CBPE_HUMAN
NCBI Protein Information
carboxypeptidase E; CPH; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase

NCBI Summary for CPE

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008]
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UniProt Comments for CPE

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; EC 3.4.17.10; Secreted

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus

Molecular Function: zinc ion binding; carboxypeptidase activity; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity

Biological Process: insulin processing; cellular protein metabolic process; neuropeptide signaling pathway; metabolic process; protein modification process; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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