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VILIP Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248508

MBS248508 is an antibody targeting VILIP. Also known as Chicken Polyclonal (IgY) to Human VILIP / VSNL1. Reactive in Bovine, Human, Mouse, Rat. Host: Chicken. More details

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Product Overview

Product Name
VILIP / VSNL1, Polyclonal Antibody
Full Product Name
Chicken Polyclonal (IgY) to Human VILIP / VSNL1
Product Gene Name
Product Synonym Gene Name
VSNL1
Product Synonym Names
Anti-VILIP / VSNL1 Antibody IHC-plus; VSNL1; Hippocalcin-like protein 3; HLP3; HUVISL1; HPCAL3; Visinin-like 1; VISL1; VILIP; VILIP-1; Visinin-like protein 1; VLP-1; Human VILIP; VSNL1
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Bovine, Human, Mouse, Rat
Form/Format
10 mM sodium azide.
Purity/Purification
Purified
Concentration
17.8 mg/ml (lot specific)
Specificity
The antibody is known to react with VLP-1 from human, cow, mouse and rat. Since VLP-1 is highly conserved in primary sequence, it is likely that the antibody is effective on other species also.
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
22,142 Da
OMIM
600817
3D Structure
ModBase 3D Structure for P62760
Target Species
Human
Immunogen Description
Recombinant full length His-tagged VLP-1 purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
VILIP / VSNL1 antibody was raised against recombinant full length His-tagged VLP-1 purified from E Coli.
Applications Tested/Suitable for anti-VILIP antibody
Immunohistochemistry (IHC), Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-VILIP antibody
IF (1:1000 - 1:2000), IHC (1:1000 - 1:2000), IHC-P (1:500), WB (1:5000 - 1:10000)
Usage: The antibody solution can be used at dilutions of 1:1000-1:2000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5000-1:10000.

Immunohistochemistry (IHC) of anti-VILIP antibody

Anti-VILIP / VSNL1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Chicken Anti-VILIP Polyclonal Antibody – Cat# MBS248508

Immunohistochemistry (IHC) of anti-VILIP antibody

Confocal image of adult rat cerebellar cortex stained with VSNL1 (green), polyclonal antibody to NF-M (red) and DNA (blue). The VSNL1 reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) and dendrites and axons are revealed with NF-M antibody. Little staining of VSNL1 is seen in the white matter (WM).
Chicken Anti-VILIP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS248508

Western Blot (WB) of anti-VILIP antibody

Western blot of rat brain homogenate stained with VSNL1. Note the strong clean band running at 18 kDa.
Chicken Anti-VILIP Polyclonal Antibody, Western Blot (WB) – Cat# MBS248508

Other Notes

Small volumes of anti-VILIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VILIP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
visinin-like protein 1
NCBI Official Synonym Full Names
visinin-like 1
NCBI Official Symbol
VSNL1
NCBI Official Synonym Symbols
HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
UniProt Protein Name
Visinin-like protein 1
UniProt Synonym Protein Names
Hippocalcin-like protein 3; HLP3
UniProt Gene Name
VSNL1
UniProt Synonym Gene Names
VISL1; VILIP; VLP-1; HLP3
UniProt Entry Name
VISL1_HUMAN
NCBI Protein Information
visinin-like protein 1; VLP-1; hippocalcin-like protein 3

NCBI Summary for VILIP

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for VILIP

VSNL1: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. Belongs to the recoverin family.

Chromosomal Location of Human Ortholog: 2p24.3

Molecular Function: calcium ion binding

Biological Process: calcium-mediated signaling

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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