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ARR3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248558

MBS248558 is an antibody targeting ARR3. Also known as Rabbit Polyclonal (IgG) to Human ARR3 / Cone Arrestin. Reactive in Human. Host: Rabbit. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARR3 / Cone Arrestin, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human ARR3 / Cone Arrestin
Product Gene Name
anti-ARR3 antibody
Product Synonym Names
ARR3 | Arrestin 4 | Arrestin-C | ARRX | C-arrestin | CArr | Cone arrestin | Retinal cone arrestin-3 | X-arrestin | CAR
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Purity/Purification
Immunoaffinity purification
Concentration
1mg/ml (lot specific)
Specificity
ARR3 antibody detects endogeneous levels of total ARR3 protein.
Preparation and Storage
Store at -20°C.
Molecular Weight
42 kDa
Immunogen Sequence Positions
aa339-388
OMIM
301770
3D Structure
ModBase 3D Structure for P36575
Target
ARR3 / Cone Arrestin
Immunogen Species
ARR3/Cone Arrestin antibody was raised against Human
Immunogen
ARR3/Cone Arrestin antibody was raised against synthetic peptide from human ARRC. (AA Range: 339-388)
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-ARR3 antibody
Western Blot (WB)
Application Notes for anti-ARR3 antibody
IHC-Paraffin: 1:50
WB: 1:500-1:1000
Peptide Enzyme-Linked Immunosorbent Assay: 1:5000

Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-ARR3 antibody

Anti-ARR3 / Cone Arrestin antibody IHC staining of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-ARR3 Polyclonal Antibody – Cat# MBS248558

Western Blot (WB) of anti-ARR3 antibody

Western blot of extracts from LOVO cells, using ARRC Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-ARR3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248558

Other Notes

Small volumes of anti-ARR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARR3. It may not necessarily be applicable to this product.

NCBI GI #
156071467
NCBI GeneID
407
NCBI Accession #
NP_004303.2
UniProt Primary Accession #
P36575
UniProt Secondary Accession #
Q5JT23; Q5JT24; Q6IBF5; Q96EN2; B5B0B9
UniProt Related Accession #
P36575
GenBank Nucleotide #
NM_004312.2
NCBI Official Full Name
arrestin-C
NCBI Official Synonym Full Names
arrestin 3, retinal (X-arrestin)
NCBI Official Symbol
ARR3
NCBI Official Synonym Symbols
ARRX
UniProt Protein Name
Arrestin-C
UniProt Synonym Protein Names
Cone arrestin; C-arrestin; cArr; Retinal cone arrestin-3; X-arrestin
Protein Family
Arrestin
UniProt Gene Name
ARR3
UniProt Synonym Gene Names
ARRX; CAR; C-arrestin; cArr
UniProt Entry Name
ARRC_HUMAN
NCBI Protein Information
arrestin-C; cArr; C-arrestin; X-arrestin; arrestin 4; cone arrestin; retinal cone arrestin-3

UniProt Comments for ARR3

ARR3: May play a role in an as yet undefined retina-specific signal transduction. Could binds to photoactivated-phosphorylated red/green opsins. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xcen-q21

Cellular Component: photoreceptor inner segment; photoreceptor outer segment; cytoplasm; synapse

Molecular Function: protein binding; opsin binding; phosphoprotein binding

Biological Process: regulation of protein amino acid phosphorylation; visual perception; endocytosis; signal transduction
Show more

Research Articles on ARR3

1. The 25-amino acid N-domain element of arrestin 3 has the highest affinity for JNK3alpha2, suggesting that it is the key site for JNK3alpha2 docking.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARR3 antibody

 Reference Product  PubMed Publications
 OPN1SW antibody  >2 publications with ARR3 and OPN1SW

Pathways associated with anti-ARR3 antibody

 Products by Pathway  Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Thromboxane A2 Receptor Signaling Pathway antibodies  Thromboxane A2 Receptor Signaling Pathway Diagram
 Visual Signal Transduction: Cones Pathway antibodies  Visual Signal Transduction: Cones Pathway Diagram
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