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NF-L Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248565

MBS248565 is an antibody targeting NF-L. Also known as Chicken Polyclonal (IgY) to Human NF-L / NEFL. Reactive in Human. Host: Chicken. More details

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Product Overview

Product Name
NF-L / NEFL, Polyclonal Antibody
Full Product Name
Chicken Polyclonal (IgY) to Human NF-L / NEFL
Product Gene Name
anti-NF-L antibody
Product Synonym Gene Name
NEFL
Product Synonym Names
Anti-NF-L / NEFL Antibody IHC-plus; NEFL; 68 kDa neurofilament protein; CMT1F; CMT2E; NF68; NF-L; NFL; Neurofilament subunit NF-L; Human NF-L; NEFL
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human
Form/Format
10 mM sodium azide.
Purity/Purification
Purified
Concentration
21.5 mg/ml (lot specific)
Specificity
Human NF-L / NEFL
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
61,517 Da
OMIM
phenotype 607734
3D Structure
ModBase 3D Structure for P07196
Target Species
Human
Immunogen Description
To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea.
Immunogen Type
Purified protein
Immunogen
NF-L / NEFL antibody was raised against to raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.
Applications Tested/Suitable for anti-NF-L antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-NF-L antibody
ICC (1:5000), IF (1:5000), IHC-P (1:500), WB (1:10000)
Usage: The IgY solution can be used at dilutions of at least 1:5000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

Immunohistochemistry (IHC) of anti-NF-L antibody

Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Chicken Anti-NF-L Polyclonal Antibody – Cat# MBS248565

Immunocytochemistry (ICC) of anti-NF-L antibody

View of mixed neuron/glial cultures stained with NF-L / NEFL antibody (red) and rabbit antibody to phosphorylated NF-H (green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.
Chicken Anti-NF-L Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS248565

Western Blot (WB) of anti-NF-L antibody

Western blots of whole protein extracts of rat spinal cord (lane 1), brain stem (lane 2), cerebellum (lane 3) and cerebral cortex (lane 4).
Chicken Anti-NF-L Polyclonal Antibody, Western Blot (WB) – Cat# MBS248565

Other Notes

Small volumes of anti-NF-L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NF-L. It may not necessarily be applicable to this product.

NCBI GI #
105990539
NCBI GeneID
4747
NCBI Accession #
NP_006149.2
UniProt Primary Accession #
P07196
UniProt Secondary Accession #
Q16154; Q8IU72; B9ZVN2
UniProt Related Accession #
P07196
GenBank Nucleotide #
NM_006158.4
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
UniProt Protein Name
Neurofilament light polypeptide
UniProt Synonym Protein Names
68 kDa neurofilament protein; Neurofilament triplet L protein
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
UniProt Entry Name
NFL_HUMAN
NCBI Protein Information
neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subunit 110

NCBI Summary for NF-L

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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UniProt Comments for NF-L

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; neuromuscular process controlling balance; locomotion; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
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Research Articles on NF-L

1. expression of NEFL induces cancer cell apoptosis and inhibits invasion in these cell lines, suggesting that NEFL may play a role in cancer cell apoptosis and invasion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NF-L antibody

 Reference Product  PubMed Publications
 MTMR2 antibody  >6 publications with NF-L and MTMR2
 GAN antibody  >3 publications with NF-L and GAN
 NEFH antibody  >3 publications with NF-L and NEFH
 NEFM antibody  >2 publications with NF-L and NEFM
 TSC1 antibody  >1 publications with NF-L and TSC1

Pathways associated with anti-NF-L antibody

 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Post NMDA Receptor Activation Events Pathway antibodies  Post NMDA Receptor Activation Events Pathway Diagram
 Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway antibodies  Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway Diagram

Diseases associated with anti-NF-L antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >54 publications with NF-L and Nervous System Diseases
 Nervous System Malformations Antibodies  >43 publications with NF-L and Nervous System Malformations
 Breast Neoplasms Antibodies  >8 publications with NF-L and Breast Neoplasms
 Brain Diseases Antibodies  >5 publications with NF-L and Brain Diseases
 Disease Models, Animal Antibodies  >4 publications with NF-L and Disease Models, Animal
 Pain Antibodies  >3 publications with NF-L and Pain
 Charcot-Marie-Tooth disease, Type 1F Antibodies  >3 publications with NF-L and Charcot-Marie-Tooth disease, Type 1F
 Lung Neoplasms Antibodies  >2 publications with NF-L and Lung Neoplasms
 Hypertension Antibodies  >2 publications with NF-L and Hypertension
 Ataxia Antibodies  >2 publications with NF-L and Ataxia

Organs/Tissues associated with anti-NF-L antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >36 publications with NF-L and Nerve
 Brain Antibodies  >17 publications with NF-L and Brain
 Prostate Antibodies  >11 publications with NF-L and Prostate
 Spinal Cord Antibodies  >8 publications with NF-L and Spinal Cord
 Lung Antibodies  >5 publications with NF-L and Lung
 Eye Antibodies  >3 publications with NF-L and Eye
 Ganglia Antibodies  >2 publications with NF-L and Ganglia
 Kidney Antibodies  >2 publications with NF-L and Kidney
 Bone Marrow Antibodies  >1 publications with NF-L and Bone Marrow
 Embryonic Tissue Antibodies  >1 publications with NF-L and Embryonic Tissue
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