• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GRG4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248567

MBS248567 is an antibody targeting GRG4. Also known as Rabbit Polyclonal (IgG) to Human GRG4 / TLE4. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
GRG4 / TLE4, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human GRG4 / TLE4
Product Gene Name
anti-GRG4 antibody
Product Synonym Gene Name
TLE4
Product Synonym Names
Anti-GRG4 / TLE4 Antibody (aa161-210) IHC-plus; TLE4; BCE1; BCE-1; E(spI); Enhancer of split groucho 4; ESG; ESG4; GRG4; Protein BCE-1; KIAA1261; R-esp2; B lymphocyte gene 1; Human GRG4; TLE4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
TLE4 Antibody detects endogenous levels of total TLE4 protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
81,011 Da
OMIM
605132
3D Structure
ModBase 3D Structure for Q04727
Target Species
Human
Immunogen Description
Synthetic peptide from human TLE4 (aa161-210).
Immunogen
GRG4 / TLE4 antibody was raised against synthetic peptide from human TLE4 (aa161-210).
Antigen Modification
aa161-210
Applications Tested/Suitable for anti-GRG4 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GRG4 antibody
ELISA (1:20000), IHC-P (20 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-GRG4 antibody

Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Rabbit Anti-GRG4 Polyclonal Antibody – Cat# MBS248567

Western Blot (WB) of anti-GRG4 antibody

Western blot of extracts from Jurkat/293/HUVEC cells, using TLE4 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-GRG4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248567

Other Notes

Small volumes of anti-GRG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRG4. It may not necessarily be applicable to this product.

NCBI GI #
34915994
NCBI GeneID
7091
NCBI Accession #
NP_008936.2
UniProt Primary Accession #
Q04727
UniProt Secondary Accession #
Q3ZCS1; Q5T1Y2; Q6PCB3; Q9BZ07; Q9BZ08; Q9BZ09; Q9NSL3; Q9ULF9; F8W6T6
UniProt Related Accession #
Q04727
GenBank Nucleotide #
NM_007005.4
NCBI Official Full Name
transducin-like enhancer protein 4 isoform 3
NCBI Official Synonym Full Names
transducin-like enhancer of split 4
NCBI Official Symbol
TLE4
NCBI Official Synonym Symbols
ESG; BCE1; ESG4; GRG4; BCE-1; Grg-4; E(spI)
UniProt Protein Name
Transducin-like enhancer protein 4
UniProt Synonym Protein Names
Grg-4; Groucho-related protein 4
UniProt Gene Name
TLE4
UniProt Synonym Gene Names
GRG4; KIAA1261
UniProt Entry Name
TLE4_HUMAN
NCBI Protein Information
transducin-like enhancer protein 4; B lymphocyte gene 1; groucho-related protein 4; enhancer of split groucho 4; transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 4, homolog of Drosophila E(sp1)

UniProt Comments for GRG4

TLE4: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. In all tissues examined, mostly in brain, and muscle. Belongs to the WD repeat Groucho/TLE family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q21.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; Notch signaling pathway; Wnt receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter
Show more

Research Articles on GRG4

1. Groucho related gene Grg4 robustly activates the expression of a BMP reporter gene, as well as enhancing and sustaining the upregulation of the endogenous Id1 gene induced by BMP7.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GRG4 antibody

 Products by Pathway  Pathway Diagram
 AMER1 Mutants Destabilize The Destruction Complex Pathway antibodies  AMER1 Mutants Destabilize The Destruction Complex Pathway Diagram
 APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway antibodies  APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway Diagram
 APC Truncation Mutants Have Impaired AXIN Binding Pathway antibodies  APC Truncation Mutants Have Impaired AXIN Binding Pathway Diagram
 AXIN Missense Mutants Destabilize The Destruction Complex Pathway antibodies  AXIN Missense Mutants Destabilize The Destruction Complex Pathway Diagram
 AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway antibodies  AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway Diagram
 Degradation Of Beta-catenin By The Destruction Complex Pathway antibodies  Degradation Of Beta-catenin By The Destruction Complex Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 NOTCH1 Intracellular Domain Regulates Transcription Pathway antibodies  NOTCH1 Intracellular Domain Regulates Transcription Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram

Diseases associated with anti-GRG4 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >3 publications with GRG4 and Carcinoma
 Neoplasms, Experimental Antibodies  >1 publications with GRG4 and Neoplasms, Experimental
 Hypertrophy Antibodies  >1 publications with GRG4 and Hypertrophy
 Lymphoma Antibodies  >1 publications with GRG4 and Lymphoma
 Fibrosis Antibodies  >1 publications with GRG4 and Fibrosis
 Adenocarcinoma Antibodies  >1 publications with GRG4 and Adenocarcinoma
 Precancerous Conditions Antibodies  >1 publications with GRG4 and Precancerous Conditions
 Congenital Abnormalities Antibodies  >1 publications with GRG4 and Congenital Abnormalities
 Adenoma Antibodies  >1 publications with GRG4 and Adenoma
 Inflammation Antibodies  >1 publications with GRG4 and Inflammation

Organs/Tissues associated with anti-GRG4 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with GRG4 and Brain
 Embryonic Tissue Antibodies  >8 publications with GRG4 and Embryonic Tissue
 Blood Antibodies  >5 publications with GRG4 and Blood
 Nerve Antibodies  >5 publications with GRG4 and Nerve
 Kidney Antibodies  >5 publications with GRG4 and Kidney
 Bone Antibodies  >4 publications with GRG4 and Bone
 Eye Antibodies  >3 publications with GRG4 and Eye
 Bone Marrow Antibodies  >2 publications with GRG4 and Bone Marrow
 Skin Antibodies  >2 publications with GRG4 and Skin
 Lung Antibodies  >2 publications with GRG4 and Lung
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000