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MAPK6 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248582

ERK3, a MAPK type protein kinase, has been associated with the ERK MAPKs based on similarity of the catalytic domain. Compared to ERK1/2, ERK 3 lacks the activating tyrosine phosphorylation site and contains only a serine which is phosphorylated by MEK. ERK3… More details

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Product Overview

Product Name
MAPK6 / ERK3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human MAPK6 / ERK3
Product Gene Name
Product Synonym Gene Name
ERK3
Product Synonym Names
Anti-MAPK6 / ERK3 Antibody (C-Terminus) IHC-plus; MAPK6; ERK-3; MAP kinase 6; MAPK 6; p97-MAPK; PRKM6; MAP kinase isoform p97; ERK3; HsT17250; p97MAPK; Human MAPK6; ERK3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bovine, Chicken, Dog, Hamster, Horse, Human, Mouse, Pig, Rabbit
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4; NP_081694.1).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
82,681 Da
OMIM
602904
3D Structure
ModBase 3D Structure for Q16659
Target Species
Human
Immunogen Description
Synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1).
Immunogen Type
Synthetic peptide
Immunogen
MAPK6 / ERK3 antibody was raised against synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%); Rat, Xenopus (92%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-MAPK6 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-MAPK6 antibody
ELISA (1:128000), IHC-P (10 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No...

Immunohistochemistry (IHC) of anti-MAPK6 antibody

Anti-MAPK6 / ERK3 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Goat Anti-MAPK6 Polyclonal Antibody – Cat# MBS248582

Western Blot (WB) of anti-MAPK6 antibody

MAPK6 antibody (1.5 ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
Goat Anti-MAPK6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248582

Western Blot (WB) of anti-MAPK6 antibody

MAPK6 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with (0.5 ug/ml) in left panel and with anti-GFP in right panel. GFP-only expression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
Goat Anti-MAPK6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248582

Other Notes

Small volumes of anti-MAPK6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAPK6 purchase

MBS248582 is an antibody targeting MAPK6. Also known as Goat Polyclonal to Human MAPK6 / ERK3. Reactive in Bovine, Chicken, Dog, Hamster, Horse, Human, Mouse, Pig, Rabbit. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPK6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase 6
NCBI Official Synonym Full Names
mitogen-activated protein kinase 6
NCBI Official Symbol
MAPK6
NCBI Official Synonym Symbols
ERK3; PRKM6; p97MAPK; HsT17250
UniProt Protein Name
Mitogen-activated protein kinase 6
UniProt Synonym Protein Names
Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
UniProt Gene Name
MAPK6
UniProt Synonym Gene Names
ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
UniProt Entry Name
MK06_HUMAN
NCBI Protein Information
mitogen-activated protein kinase 6; ERK-3; MAPK 6; p97-MAPK; MAP kinase 6; MAP kinase isoform p97; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97

NCBI Summary for MAPK6

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAPK6

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; ATP binding

Biological Process: MAPKKK cascade; cell cycle; signal transduction; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAPK6 antibody

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