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TRAM1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248608

MBS248608 is an antibody targeting TRAM1. Also known as Rabbit Polyclonal to Human TRAM1. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TRAM1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human TRAM1
Product Gene Name
Product Synonym Names
Anti-TRAM1 Antibody (aa310-374) IHC-plus; TRAM1; PNAS8; TRAM; TRAMP; Human TRAM1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human TRAM1
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.
Molecular Weight
39,683 Da
OMIM
605190
3D Structure
ModBase 3D Structure for Q15629
Target Species
Human
Immunogen Description
Synthetic peptide corresponding to a region within amino acids 310 and 374 of TRAM1 (SwissProt Q15629).
Immunogen Type
Synthetic peptide
Immunogen
TRAM1 antibody was raised against synthetic peptide corresponding to a region within amino acids 310 and 374 of TRAM1 (SwissProt Q15629).
Antigen Modification
aa310-374
Applications Tested/Suitable for anti-TRAM1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-TRAM1 antibody
ICC (1:100 - 1:1000), IHC-P (1:100), WB (1:500 - 1:3000)
Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Immunohistochemistry (IHC) of anti-TRAM1 antibody

Anti-TRAM1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-TRAM1 Polyclonal Antibody – Cat# MBS248608

Immunohistochemistry (IHC) of anti-TRAM1 antibody

Anti-TRAM1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-TRAM1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS248608

Immunocytochemistry (ICC) of anti-TRAM1 antibody

Immunofluorescence of methanol-fixed HeLa using TRAM1 antibody at 1:500 dilution.
Rabbit Anti-TRAM1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS248608

Western Blot (WB) of anti-TRAM1 antibody

Sample (30 ug of whole cell lysate). A: H1299. 10% SDS PAGE. TRAM1 antibody diluted at 1:1000.
Rabbit Anti-TRAM1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248608

Other Notes

Small volumes of anti-TRAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRAM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
translocating chain-associated membrane protein 1
NCBI Official Synonym Full Names
translocation associated membrane protein 1
NCBI Official Symbol
TRAM1
NCBI Official Synonym Symbols
TRAM; PNAS8; TRAMP
UniProt Protein Name
Translocating chain-associated membrane protein 1
UniProt Gene Name
TRAM1
UniProt Synonym Gene Names
TRAM
UniProt Entry Name
TRAM1_HUMAN
NCBI Protein Information
translocating chain-associated membrane protein 1; translocation-associating membrane protein 1; translocating chain-associating membrane protein

NCBI Summary for TRAM1

This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]
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UniProt Comments for TRAM1

TRAM1: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Belongs to the TRAM family.

Protein type: Membrane protein, multi-pass; Receptor, misc.; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; receptor activity

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; viral reproduction; gene expression; cotranslational protein targeting to membrane
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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