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DCI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248636

MBS248636 is an antibody targeting DCI. Also known as Rabbit Polyclonal to Human DCI / ECI1. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DCI / ECI1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human DCI / ECI1
Product Gene Name
Product Synonym Gene Name
ECI1
Product Synonym Names
Anti-DCI / ECI1 Antibody (aa273-302) IHC-plus; ECI1; 3;2 trans-enoyl-CoA isomerase; 3;2-trans-enoyl-CoA isomerase; Acetylene-allene isomerase; DCI; Dodecenoyl-CoA isomerase; Enoyl-CoA delta isomerase 1; D3;D2-enoyl-CoA isomerase; Human DCI; ECI1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Specificity
Human DCI / ECI1
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
30,896 Da
OMIM
600305
Target Species
Human
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human DCI.
Immunogen Type
Synthetic peptide
Immunogen
DCI / ECI1 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human DCI.
Antigen Modification
aa273-302
Applications Tested/Suitable for anti-DCI antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-DCI antibody
Flo (1:10 - 1:50), IHC-P (1:10 - 1:100), WB (1:1000)

Immunohistochemistry (IHC) of anti-DCI antibody

Anti-DCI / ECI1 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-DCI Polyclonal Antibody – Cat# MBS248636

Flow Cytometry (FC/FACS) of anti-DCI antibody

DCI Antibody (C-term) flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-DCI Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS248636

Western Blot (WB) of anti-DCI antibody

Western blot of DCI Antibody (C-term) in mouse heart tissue and MCF-7, HL-60 cell line lysates (35 ug/lane). DCI (arrow) was detected using the purified Pab.
Rabbit Anti-DCI Polyclonal Antibody, Western Blot (WB) – Cat# MBS248636

Other Notes

Small volumes of anti-DCI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
enoyl-CoA delta isomerase 1, mitochondrial isoform 1
NCBI Official Synonym Full Names
enoyl-CoA delta isomerase 1
NCBI Official Symbol
ECI1
NCBI Official Synonym Symbols
DCI
UniProt Protein Name
Enoyl-CoA delta isomerase 1, mitochondrial
UniProt Synonym Protein Names
3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase
UniProt Gene Name
ECI1
UniProt Synonym Gene Names
DCI; D3,D2-enoyl-CoA isomerase
UniProt Entry Name
ECI1_HUMAN
NCBI Protein Information
enoyl-CoA delta isomerase 1, mitochondrial; dodecenoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; acetylene-allene isomerase; 3,2 trans-enoyl-CoA isomerase; delta3, delta2-enoyl-CoA isomerase; 3,2 trans-enoyl-Coenzyme A isomerase; delta(3),Delta(2)-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)

NCBI Summary for DCI

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
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UniProt Comments for DCI

DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; Mitochondrial; EC 5.3.3.8; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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