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DCUN1D1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248657

MBS248657 is an antibody targeting DCUN1D1. Also known as Goat Polyclonal to Human DCUN1D1 / SCCRO. Reactive in Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Horse, Human, Monkey, Mouse, Rabbit. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DCUN1D1 / SCCRO, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human DCUN1D1 / SCCRO
Product Gene Name
Product Synonym Gene Name
SCCRO
Product Synonym Names
Anti-DCUN1D1 / SCCRO Antibody (C-Terminus) IHC-plus; DCUN1D1; DCN1-like protein 1; DCNL1; DCUN1L1; RP42 homolog; RP42; Tes3; SCCRO; SCRO; Human DCUN1D1; SCCRO
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Horse, Human, Monkey, Mouse, Rabbit
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human DCUN1D1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
30,124 Da
OMIM
605905
3D Structure
ModBase 3D Structure for Q96GG9
Target Species
Human
Immunogen Description
Synthetic peptide C-RPQIAGTKSTT from the C-terminus of human DCUN1D1 (NP_065691.2).
Immunogen Type
Synthetic peptide
Immunogen
DCUN1D1 / SCCRO antibody was raised against synthetic peptide C-RPQIAGTKSTT from the C-terminus of human DCUN1D1 (NP_065691.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Chicken, Platypus, Xenopus (100%); Rat, Opossum, Lizard (91%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-DCUN1D1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-DCUN1D1 antibody
ELISA (1:16000), IHC-P (5 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 28kDa band observed in HepG2 lysates (calculated MW of 30.1kDa according to NP_065691.2). Recommended concentration: 0.3-1 ug/ml.

Immunohistochemistry (IHC) of anti-DCUN1D1 antibody

Anti-DCUN1D1 / SCCRO antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-DCUN1D1 Polyclonal Antibody – Cat# MBS248657

Western Blot (WB) of anti-DCUN1D1 antibody

DCUN1D1 antibody (0.5 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DCUN1D1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248657

Other Notes

Small volumes of anti-DCUN1D1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCUN1D1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DCN1-like protein 1
NCBI Official Synonym Full Names
DCN1, defective in cullin neddylation 1, domain containing 1
NCBI Official Symbol
DCUN1D1
NCBI Official Synonym Symbols
RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1
UniProt Protein Name
DCN1-like protein 1
UniProt Synonym Protein Names
DCUN1 domain-containing protein 1; Defective in cullin neddylation protein 1-like protein 1; Squamous cell carcinoma-related oncogene
Protein Family
UniProt Gene Name
DCUN1D1
UniProt Synonym Gene Names
DCUN1L1; RP42; SCCRO
UniProt Entry Name
DCNL1_HUMAN
NCBI Protein Information
DCN1-like protein 1; RP42 homolog; DCUN1 domain-containing protein 1; squamous cell carcinoma related oncogene; squamous cell carcinoma-related oncogene; defective in cullin neddylation protein 1-like protein 1

UniProt Comments for DCUN1D1

DCUN1D1: Part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression.

Protein type: Oncoprotein; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: ubiquitin ligase complex

Molecular Function: protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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