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THNSL2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248677

MBS248677 is an antibody targeting THNSL2. Also known as Goat Polyclonal to Human THNSL2. Reactive in Human. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
THNSL2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human THNSL2
Product Gene Name
Product Synonym Names
Anti-THNSL2 Antibody (aa192-204) IHC-plus; THNSL2; Threonine synthase-like 2; THS2; TSH2; SOFAT; Human THNSL2
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human THNSL2. This antibody is expected to recognize all reported isoforms (NP_060741.3; NP_001231605.1; NP_001231607.1).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
27,414 Da
OMIM
611261
3D Structure
ModBase 3D Structure for Q86YJ6
Target Species
Human
Immunogen Description
Synthetic peptide C-EGNSDELDEPIKT from an internal region of human THNSL2 (NP_060741.3; NP_001231605.1; NP_001231607.1). Percent identity by BLAST analysis: Hamster, Panda, Dog, Horse, Guinea pig (92%); Cat, Rabbit, Platypus, Lizard (85%).
Immunogen Type
Synthetic peptide
Immunogen
THNSL2 antibody was raised against synthetic peptide C-EGNSDELDEPIKT from an internal region of human THNSL2 (NP_060741.3; NP_001231605.1; NP_001231607.1). Percent identity by BLAST analysis: Hamster, Panda, Dog, Horse, Guinea pig (92%); Cat, Rabbit, Platypus, Lizard (85%).
Antigen Modification
aa192-204
Applications Tested/Suitable for anti-THNSL2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-THNSL2 antibody
ELISA (1:128000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 40kD band observed in Human Colorectal cancer lysates (calculated MW of 45.0kD according to NP_001231605.1). Recommended concentration: 1-3 ug/ml.

Immunohistochemistry (IHC) of anti-THNSL2 antibody

Anti-THNSL2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-THNSL2 Polyclonal Antibody – Cat# MBS248677

Western Blot (WB) of anti-THNSL2 antibody

THNSL2 antibody (2 ug/ml) staining of Human Colorectal Cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-THNSL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248677

Other Notes

Small volumes of anti-THNSL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for THNSL2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
threonine synthase-like 2 isoform 1
NCBI Official Synonym Full Names
threonine synthase-like 2 (S. cerevisiae)
NCBI Official Symbol
THNSL2
NCBI Official Synonym Symbols
THS2; TSH2; SOFAT
UniProt Protein Name
Threonine synthase-like 2
UniProt Synonym Protein Names
Secreted osteoclastogenic factor of activated T-cells; SOFAT
Protein Family
UniProt Gene Name
THNSL2
UniProt Synonym Gene Names
TSH2; SOFAT
UniProt Entry Name
THNS2_HUMAN
NCBI Protein Information
threonine synthase-like 2; secreted osteoclastogenic factor of activated T cells

NCBI Summary for THNSL2

This gene encodes a threonine synthase-like protein. A similar enzyme in mouse can catalyze the degradation of O-phospho-homoserine to a-ketobutyrate, phosphate, and ammonia. This protein also has phospho-lyase activity on both gamma and beta phosphorylated substrates. In mouse an alternatively spliced form of this protein has been shown to act as a cytokine and can induce the production of the inflammatory cytokine IL6 in osteoblasts. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
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UniProt Comments for THNSL2

THNSL2: Isoform 1: Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho- threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate. Belongs to the threonine synthase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.3.-; Lyase

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: cytokine activity; threonine synthase activity; pyridoxal phosphate binding

Biological Process: dephosphorylation; 2-oxobutyrate biosynthetic process; threonine biosynthetic process; serine family amino acid catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-THNSL2 antibody

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