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PH Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248716

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PH / PAH, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human PH / PAH
Product Gene Name
Product Synonym Gene Name
PAH
Product Synonym Names
Anti-PH / PAH Antibody (Internal) IHC-plus; PAH; Phe-4-monooxygenase; PKU1; PH; Phenylalanine 4-monooxygenase; Phe-4- monooxygenase; Phenylalanine hydroxylase; Phenylalanine-4-hydroxylase; PKU; Human PH; PAH
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Dog, Gorilla, Horse, Human, Monkey, Orangutan, Rabbit
Predicted Reactivity: Mouse, Rat, Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PAH / Phenylalanine Hydroxylase.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
51,862 Da
OMIM
phenotype 612349
3D Structure
ModBase 3D Structure for P00439
Target Species
Human
Immunogen Description
Synthetic peptide C-ESRPSRLKKDE from an internal region of human PAH / Phenylalanine Hydroxylase (NP_000268.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%).
Immunogen Type
Synthetic peptide
Immunogen
PH / PAH antibody was raised against synthetic peptide C-ESRPSRLKKDE from an internal region of human PAH / Phenylalanine Hydroxylase (NP_000268.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%); Mouse, Rat, Bovine, Turkey, Chicken, Lizard, Xenopus (91%); Hamster, Opossum, Platypus, Drosophila, Nematode (82%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-PH antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PH antibody
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in Human Liver lysates (calculated MW of 51.9kD according to NP_000268.1). Recommended concentration: 0.3-1 ug/ml.

Immunohistochemistry (IHC) of anti-PH antibody

Anti-PH / PAH antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Goat Anti-PH Polyclonal Antibody – Cat# MBS248716

Western Blot (WB) of anti-PH antibody

PAH antibody (0.3 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PH Polyclonal Antibody, Western Blot (WB) – Cat# MBS248716

Other Notes

Small volumes of anti-PH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PH purchase

MBS248716 is an antibody targeting PH. Also known as Goat Polyclonal to Human PH / PAH. Reactive in Bat, Gibbon, Dog, Gorilla, Horse, Human, Monkey, Orangutan, Rabbit
Predicted Reactivity: Mouse, Rat, Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for PH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phenylalanine-4-hydroxylase
NCBI Official Synonym Full Names
phenylalanine hydroxylase
NCBI Official Symbol
PAH
NCBI Official Synonym Symbols
PH; PKU; PKU1
UniProt Protein Name
Phenylalanine-4-hydroxylase
UniProt Synonym Protein Names
Phe-4-monooxygenase
UniProt Gene Name
PAH
UniProt Synonym Gene Names
PAH
UniProt Entry Name
PH4H_HUMAN
NCBI Protein Information
phenylalanine-4-hydroxylase; phe-4-monooxygenase; phenylalanine 4-monooxygenase

UniProt Comments for PH

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Protein type: EC 1.14.16.1; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q22-q24.2

Cellular Component: cytosol

Molecular Function: amino acid binding; iron ion binding; phenylalanine 4-monooxygenase activity

Biological Process: L-phenylalanine catabolic process; catecholamine biosynthetic process; amino acid biosynthetic process; neurotransmitter biosynthetic process

Disease: Phenylketonuria
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

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