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MCF2L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248723

MBS248723 is an antibody targeting MCF2L. Also known as Rabbit Polyclonal to Human MCF2L / DBS. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MCF2L / DBS, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human MCF2L / DBS
Product Gene Name
anti-MCF2L antibody
Product Synonym Gene Name
DBS
Product Synonym Names
Anti-MCF2L / DBS Antibody (aa1048-1075) IHC-plus; MCF2L; ARHGEF14; DBS; KIAA0362; OST; DBLs big sister; Human MCF2L; DBS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Specificity
Human MCF2L / DBS
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
126,111 Da
OMIM
609499
3D Structure
ModBase 3D Structure for O15068
Target Species
Human
Immunogen Description
KLH-conjugated synthetic selected from C-terminal region of human MCF2L.
Immunogen
MCF2L / DBS antibody was raised against kLH-conjugated synthetic selected from C-terminal region of human MCF2L.
Antigen Modification
aa1048-1075
Applications Tested/Suitable for anti-MCF2L antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-MCF2L antibody
IHC-P (1:100), WB (1:1000)

Immunohistochemistry (IHC) of anti-MCF2L antibody

Anti-MCF2L / DBS antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-MCF2L Polyclonal Antibody – Cat# MBS248723

Western Blot (WB) of anti-MCF2L antibody

MCF2L Antibody (C-term) Western blot of NCI-H460 cell line lysates (35 ug/lane). This demonstrates the MCF2L antibody detected the MCF2L protein (arrow).
Rabbit Anti-MCF2L Polyclonal Antibody, Western Blot (WB) – Cat# MBS248723

Other Notes

Small volumes of anti-MCF2L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCF2L. It may not necessarily be applicable to this product.

NCBI GI #
163644323
NCBI GeneID
23263
NCBI Accession #
NP_079255.3
UniProt Primary Accession #
O15068
UniProt Secondary Accession #
Q5JU56; Q5VXT1; Q6ZWD4; Q765G8; A2A2X1; A2A2X2; A2A3G6; A2A3G8; B4DHD6; B4DIL6; E9PDN8
UniProt Related Accession #
O15068
GenBank Nucleotide #
NM_024979.3
NCBI Official Full Name
guanine nucleotide exchange factor DBS isoform b
NCBI Official Synonym Full Names
MCF.2 cell line derived transforming sequence-like
NCBI Official Symbol
MCF2L
NCBI Official Synonym Symbols
DBS; OST; ARHGEF14
UniProt Protein Name
Guanine nucleotide exchange factor DBS
UniProt Synonym Protein Names
DBL's big sister; MCF2-transforming sequence-like protein
Protein Family
Guanine nucleotide exchange factor
UniProt Gene Name
MCF2L
UniProt Synonym Gene Names
KIAA0362
UniProt Entry Name
MCF2L_HUMAN
NCBI Protein Information
guanine nucleotide exchange factor DBS; DBL's big sister; MCF2 transforming sequence-like protein

UniProt Comments for MCF2L

ARHGEF14: Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus. Isoform 5 activates CDC42. Belongs to the MCF2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; Oncoprotein; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular space; lamellipodium; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; phosphatidylinositol binding

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; positive regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction
Show more

Research Articles on MCF2L

1. In conclusion, we find that GDF5, but not COG5 or MCF2L, influence the extent of radiographic damage in knee osteoarthritis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MCF2L antibody

 Products by Pathway  Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 G Alpha (12/13) Signalling Events Pathway antibodies  G Alpha (12/13) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 NRAGE Signals Death Through JNK Pathway antibodies  NRAGE Signals Death Through JNK Pathway Diagram
 Neurotrophic Factor-mediated Trk Receptor Signaling Pathway antibodies  Neurotrophic Factor-mediated Trk Receptor Signaling Pathway Diagram
 Regulation Of CDC42 Activity Pathway antibodies  Regulation Of CDC42 Activity Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram
 Rho GTPase Cycle Pathway antibodies  Rho GTPase Cycle Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with anti-MCF2L antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >3 publications with MCF2L and Cell Transformation, Neoplastic
 Hypertrophy Antibodies  >1 publications with MCF2L and Hypertrophy
 Neurobehavioral Manifestations Antibodies  >1 publications with MCF2L and Neurobehavioral Manifestations
 Renal Insufficiency Antibodies  >1 publications with MCF2L and Renal Insufficiency
 Nervous System Diseases Antibodies  >1 publications with MCF2L and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with MCF2L and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with MCF2L and Kidney Diseases

Organs/Tissues associated with anti-MCF2L antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with MCF2L and Brain
 Thymus Antibodies  >2 publications with MCF2L and Thymus
 Prostate Antibodies  >2 publications with MCF2L and Prostate
 Parathyroid Antibodies  >1 publications with MCF2L and Parathyroid
 Ascites Antibodies  >1 publications with MCF2L and Ascites
 Muscle Antibodies  >1 publications with MCF2L and Muscle
 Liver Antibodies  >1 publications with MCF2L and Liver
 Intestine Antibodies  >1 publications with MCF2L and Intestine
 Kidney Antibodies  >1 publications with MCF2L and Kidney
 Bone Antibodies  >1 publications with MCF2L and Bone
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