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BLK Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248765

Protein Tyrosine Kinase BLK is a non-receptor tyrosine kinase belonging to the Src subfamily. It functions in a signal transductory pathway specific to the B-lymphoid cell lineage. As compared to the mouse, human BLK contains a unique domain with a 6 amino… More details

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Product Overview

Product Name
BLK, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human BLK
Product Gene Name
Product Synonym Names
Anti-BLK Antibody (N-Terminus) IHC-plus; BLK; Blk kinase; B lymphocyte kinase; B lymphoid tyrosine kinase; MODY11; Protein kinase blk; Protein tyrosine kinase blk; B lymphoid kinase; p55-Blk; Tyrosine-protein kinase Blk; Human BLK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Monkey
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human BLK.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
57,706 Da
OMIM
gene 613375
3D Structure
ModBase 3D Structure for P51451
Target Species
Human
Immunogen Description
Synthetic peptide QWSPLKVSAQDKD-C from the N-terminus (near) of human BLK (NP_001706.2). Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (85%).
Immunogen Type
Synthetic peptide
Immunogen
BLK antibody was raised against synthetic peptide QWSPLKVSAQDKD-C from the N-terminus (near) of human BLK (NP_001706.2). Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (85%).
Antigen Modification
N-Terminus
Applications Tested/Suitable for anti-BLK antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-BLK antibody
ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50kD band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kD according to NP_001706.2). Recommended concentration: 0.1-0.3 ug/ml.

Immunohistochemistry (IHC) of anti-BLK antibody

Anti-BLK antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Goat Anti-BLK Polyclonal Antibody – Cat# MBS248765

Western Blot (WB) of anti-BLK antibody

BLK antibody (0.1 ug/ml) staining of Human Thymus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-BLK Polyclonal Antibody, Western Blot (WB) – Cat# MBS248765

Other Notes

Small volumes of anti-BLK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BLK purchase

MBS248765 is an antibody targeting BLK. Also known as Goat Polyclonal to Human BLK. Reactive in Human, Monkey. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for BLK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine-protein kinase Blk
NCBI Official Synonym Full Names
BLK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
BLK
NCBI Official Synonym Symbols
MODY11
UniProt Protein Name
Tyrosine-protein kinase Blk
UniProt Synonym Protein Names
B lymphocyte kinase; p55-Blk
UniProt Gene Name
BLK
UniProt Entry Name
BLK_HUMAN
NCBI Protein Information
tyrosine-protein kinase Blk; p55-Blk; b lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase

NCBI Summary for BLK

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]
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UniProt Comments for BLK

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; positive regulation of insulin secretion; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation

Disease: Maturity-onset Diabetes Of The Young, Type 11
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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