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TSHZ2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248786

MBS248786 is an antibody targeting TSHZ2. Also known as Rabbit Polyclonal to Human TSHZ2. Reactive in Human. Host: Rabbit. More details

$650 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TSHZ2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human TSHZ2
Product Gene Name
Product Synonym Names
Anti-TSHZ2 Antibody (aa470-500) IHC-plus; TSHZ2; ZABC2; TSH2; OVC10-2; Zinc finger protein 218; Teashirt homolog 2; C20orf17; Teashirt family zinc finger 2; ZNF218; Human TSHZ2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Specificity
Human TSHZ2
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Target Species
Human
Immunogen Description
KLH-conjugated synthetic peptide from N-terminal region of human TSH2.
Immunogen Type
Synthetic peptide
Immunogen
TSHZ2 antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human TSH2.
Antigen Modification
aa470-500
Applications Tested/Suitable for anti-TSHZ2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-TSHZ2 antibody
Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000)

Immunohistochemistry (IHC) of anti-TSHZ2 antibody

Anti-TSHZ2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-TSHZ2 Polyclonal Antibody – Cat# MBS248786

Immunohistochemistry (IHC) of anti-TSHZ2 antibody

TSH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry.
Rabbit Anti-TSHZ2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS248786

Immunofluorescence (IF) of anti-TSHZ2 antibody

Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37[Character 2103]). For secondary antibody, Alexa Fluor[Character ae] 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37[Character 2103]).Cytoplasmic actin was counterstained with Alexa Fluor[Character ae] 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37[Character 2103]). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.
Rabbit Anti-TSHZ2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS248786

Flow Cytometry (FC/FACS) of anti-TSHZ2 antibody

TSH2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-TSHZ2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS248786

Western Blot (WB) of anti-TSHZ2 antibody

Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35 ug/lane).TSH2 (arrow) was detected using the purified Pab.
Rabbit Anti-TSHZ2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248786

Other Notes

Small volumes of anti-TSHZ2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TSHZ2. It may not necessarily be applicable to this product.

NCBI GeneID
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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