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RUFY1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248822

MBS248822 is an antibody targeting RUFY1. Also known as Goat Polyclonal to Human RUFY1. Reactive in Gibbon, Human, Monkey, Rabbit. Host: Goat. More details

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Product Overview

Product Name
RUFY1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human RUFY1
Product Gene Name
Product Synonym Names
Anti-RUFY1 Antibody (Internal) IHC-plus; RUFY1; FYVE-finger protein EIP1; RABIP4; La-binding protein 1; Rab4-interacting protein; ZFYVE12; Human RUFY1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Human, Monkey, Rabbit
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human RUFY1. This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
44,489 Da
OMIM
610327
3D Structure
ModBase 3D Structure for Q96T51
Target Species
Human
Immunogen Description
Synthetic peptide C-QKNEAITSFEGKT from an internal region of human RUFY1 (NP_079434.3; NP_001035541.1). Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Elephant, Rabbit (100%); Mouse, Hamster (92%).
Immunogen Type
Synthetic peptide
Immunogen
RUFY1 antibody was raised against synthetic peptide C-QKNEAITSFEGKT from an internal region of human RUFY1 (NP_079434.3; NP_001035541.1). Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Elephant, Rabbit (100%); Mouse, Hamster (92%); Rat, Panda, Pig, Opossum, Chicken, Platypus, Lizard (85%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-RUFY1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RUFY1 antibody
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.05 - 0.2 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 85kD band observed in lysates of cell line HeLa (calculated MW of 79.8kD according to NP_079434.3). Recommended concentration: 0.05-0.2 ug/ml.

Immunohistochemistry (IHC) of anti-RUFY1 antibody

Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Goat Anti-RUFY1 Polyclonal Antibody – Cat# MBS248822

Western Blot (WB) of anti-RUFY1 antibody

RUFY1 antibody (0.05 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-RUFY1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248822

Other Notes

Small volumes of anti-RUFY1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RUFY1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RUN and FYVE domain-containing protein 1 isoform a
NCBI Official Synonym Full Names
RUN and FYVE domain containing 1
NCBI Official Symbol
RUFY1
NCBI Official Synonym Symbols
RABIP4; ZFYVE12
UniProt Protein Name
RUN and FYVE domain-containing protein 1
UniProt Synonym Protein Names
FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12
UniProt Gene Name
RUFY1
UniProt Synonym Gene Names
RABIP4; ZFYVE12
UniProt Entry Name
RUFY1_HUMAN
NCBI Protein Information
RUN and FYVE domain-containing protein 1; la-binding protein 1; FYVE-finger protein EIP1; rab4-interacting protein; zinc finger FYVE domain-containing protein 12

NCBI Summary for RUFY1

This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for RUFY1

RUFY1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; lipid binding; protein transporter activity

Biological Process: protein transport; regulation of endocytosis; endocytosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RUFY1 antibody

Pathways associated with anti-RUFY1 antibody

Organs/Tissues associated with anti-RUFY1 antibody

 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >1 publications with RUFY1 and Ovary
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