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TRIM54 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248888

MBS248888 is an antibody targeting TRIM54. Also known as Goat Polyclonal to Human TRIM54 / MURF. Reactive in Gibbon, Dog, Gorilla, Human, Monkey, PigPredicted Reactivity: Orangutan (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TRIM54 / MURF, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human TRIM54 / MURF
Product Gene Name
Product Synonym Gene Name
MURF
Product Synonym Names
Anti-TRIM54 / MURF Antibody (C-Terminus) IHC-plus; TRIM54; MURF; MURF-3; MuRF3; RING finger protein 30; Tripartite motif-containing 54; RNF30; Tripartite motif containing 54; Human TRIM54; MURF
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Dog, Gorilla, Human, Monkey, Pig
Predicted Reactivity: Orangutan (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human TRIM54.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
44,833 Da
OMIM
606474
3D Structure
ModBase 3D Structure for Q9BYV2
Target Species
Human
Immunogen Description
Synthetic peptide C-GSAGPEEERPDGP from the C-terminus of human TRIM54 (NP_115935.3; NP_912730.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Pig (100%); Orangutan (92%); Marmoset, Elephant, Panda, Horse (85%).
Immunogen Type
Synthetic peptide
Immunogen
TRIM54 / MURF antibody was raised against synthetic peptide C-GSAGPEEERPDGP from the C-terminus of human TRIM54 (NP_115935.3; NP_912730.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Pig (100%); Orangutan (92%); Marmoset, Elephant, Panda, Horse (85%).
Antigen Modification
C-Terminus

Product Categories/Family for anti-TRIM54 antibody

Applications Tested/Suitable for anti-TRIM54 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TRIM54 antibody
ELISA (1:8000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml)

Immunohistochemistry (IHC) of anti-TRIM54 antibody

Anti-TRIM54 / MURF antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Goat Anti-TRIM54 Polyclonal Antibody – Cat# MBS248888

Western Blot (WB) of anti-TRIM54 antibody

Antibody staining (1 ug/ml) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-TRIM54 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248888

Other Notes

Small volumes of anti-TRIM54 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM54. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tripartite motif-containing protein 54 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 54
NCBI Official Symbol
TRIM54
NCBI Official Synonym Symbols
MURF; RNF30; muRF3; MURF-3
UniProt Protein Name
Tripartite motif-containing protein 54
UniProt Synonym Protein Names
Muscle-specific RING finger protein; MuRF; Muscle-specific RING finger protein 3; MuRF-3; MuRF3; RING finger protein 30
UniProt Gene Name
TRIM54
UniProt Synonym Gene Names
MURF; MURF3; RNF30; MuRF; MuRF-3; MuRF3
UniProt Entry Name
TRI54_HUMAN
NCBI Protein Information
tripartite motif-containing protein 54; ring finger protein 30; tripartite motif-containing 54; muscle-specific RING finger protein 3; muscle-specific RING-finger protein 3

NCBI Summary for TRIM54

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for TRIM54

TRIM54: May bind and stabilize microtubules during myotubes formation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: microtubule; microtubule associated complex; Z disc

Molecular Function: signal transducer activity; zinc ion binding; microtubule binding

Biological Process: negative regulation of microtubule depolymerization; multicellular organismal development; signal transduction; cell differentiation; microtubule-based process
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Research Articles on TRIM54

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRIM54 antibody

Diseases associated with anti-TRIM54 antibody

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >3 publications with TRIM54 and Atrophy

Organs/Tissues associated with anti-TRIM54 antibody

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