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MAPRE2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249016

MBS249016 is an antibody targeting MAPRE2. Also known as Mouse Monoclonal [clone 1F3] (IgG2a) to Human MAPRE2 / EB2. Reactive in Human. Host: Mouse. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAPRE2 / EB2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1F3] (IgG2a) to Human MAPRE2 / EB2
Product Gene Name
Product Synonym Gene Name
EB2
Product Synonym Names
Anti-MAPRE2 / EB2 Antibody (clone 1F3) IHC-plus; MAPRE2; APC-binding protein EB2; APC-binding protein EB1; EB1; EB2; End-binding protein 2; Human MAPRE2; EB2
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1F3
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
0.81 mg/ml (lot specific)
Specificity
Human MAPRE2 / EB2
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
30,691 Da
OMIM
605789
3D Structure
ModBase 3D Structure for Q15555
Target Species
Human
Immunogen Description
Full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
MAPRE2 / EB2 antibody was raised against full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
Applications Tested/Suitable for anti-MAPRE2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-MAPRE2 antibody
Flo (1:100), IF (1:100), IHC-P (20 ug/ml), WB (1:2000)

Immunohistochemistry (IHC) of anti-MAPRE2 antibody

Anti-MAPRE2 / EB2 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Mouse Anti-MAPRE2 Monoclonal Antibody – Cat# MBS249016

Immunofluorescence (IF) of anti-MAPRE2 antibody

Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.
Mouse Anti-MAPRE2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249016

Flow Cytometry (FC/FACS) of anti-MAPRE2 antibody

Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-MAPRE2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249016

Flow Cytometry (FC/FACS) of anti-MAPRE2 antibody

Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-MAPRE2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249016

Flow Cytometry (FC/FACS) of anti-MAPRE2 antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.
Mouse Anti-MAPRE2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249016

Western Blot (WB) of anti-MAPRE2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.
Mouse Anti-MAPRE2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249016

Other Notes

Small volumes of anti-MAPRE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPRE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
microtubule-associated protein RP/EB family member 2 isoform 1
NCBI Official Synonym Full Names
microtubule-associated protein, RP/EB family, member 2
NCBI Official Symbol
MAPRE2
NCBI Official Synonym Symbols
EB1; EB2; RP1
UniProt Protein Name
Microtubule-associated protein RP/EB family member 2
UniProt Synonym Protein Names
APC-binding protein EB2; End-binding protein 2; EB2
UniProt Gene Name
MAPRE2
UniProt Synonym Gene Names
RP1; EB2
UniProt Entry Name
MARE2_HUMAN
NCBI Protein Information
microtubule-associated protein RP/EB family member 2; end-binding protein 2; APC-binding protein EB1; APC-binding protein EB2; T-cell activation protein, EB1 family

NCBI Summary for MAPRE2

The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
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UniProt Comments for MAPRE2

RP1: shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. Its function is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: microtubule cytoskeleton; cytoplasm

Molecular Function: microtubule binding

Biological Process: cell proliferation; mitosis; cell division; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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