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PGAM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249017

MBS249017 is an antibody targeting PGAM2. Also known as Mouse Monoclonal [clone 4E9] (IgG2a) to Human PGAM2. Reactive in Human, Monkey, Mouse. Host: Mouse. More details

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Product Overview

Product Name
PGAM2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4E9] (IgG2a) to Human PGAM2
Product Gene Name
anti-PGAM2 antibody
Product Synonym Names
Anti-PGAM2 Antibody (clone 4E9) IHC-plus; PGAM2; BPG-dependent PGAM 2; GSD10; PGAMM; PGAM-M; Phosphoglycerate mutase 2; Human PGAM2
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4E9
Host
Mouse
Species Reactivity
Human, Monkey, Mouse
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human PGAM2
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
28,766 Da
OMIM
phenotype 612931
3D Structure
ModBase 3D Structure for P15259
Target Species
Human
Immunogen Description
Full length human recombinant protein of human PGAM2(NP_000028) produced in HEK293 cell.
Immunogen Type
Recombinant protein
Immunogen
PGAM2 antibody was raised against full length human recombinant protein of human PGAM2(NP_000028) produced in HEK293 cell.
Applications Tested/Suitable for anti-PGAM2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PGAM2 antibody
Flo (1:100), IF (1:50 - 1:100), IHC-P (1:150, 10 ug/ml), WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-PGAM2 antibody

Anti-PGAM2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-PGAM2 Monoclonal Antibody – Cat# MBS249017

Immunofluorescence (IF) of anti-PGAM2 antibody

Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.
Mouse Anti-PGAM2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249017

Immunofluorescence (IF) of anti-PGAM2 antibody

Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.
Mouse Anti-PGAM2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249017

Flow Cytometry (FC/FACS) of anti-PGAM2 antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody, and then analyzed by flow cytometry.
Mouse Anti-PGAM2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249017

Western Blot (WB) of anti-PGAM2 antibody

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
Mouse Anti-PGAM2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249017

Western Blot (WB) of anti-PGAM2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.
Mouse Anti-PGAM2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249017

Other Notes

Small volumes of anti-PGAM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PGAM2. It may not necessarily be applicable to this product.

NCBI GI #
50593010
NCBI GeneID
5224
NCBI Accession #
NP_000281.2
UniProt Primary Accession #
P15259
UniProt Related Accession #
P15259
GenBank Nucleotide #
NM_000290.3
NCBI Official Full Name
phosphoglycerate mutase 2
NCBI Official Synonym Full Names
phosphoglycerate mutase 2 (muscle)
NCBI Official Symbol
PGAM2
NCBI Official Synonym Symbols
GSD10; PGAMM; PGAM-M
UniProt Protein Name
Phosphoglycerate mutase 2
UniProt Synonym Protein Names
BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M; PGAM-M
Protein Family
Phosphoglycerate mutase
UniProt Gene Name
PGAM2
UniProt Synonym Gene Names
PGAMM; PGAM-M
UniProt Entry Name
PGAM2_HUMAN
NCBI Protein Information
phosphoglycerate mutase 2; BPG-dependent PGAM 2; phosphoglycerate mutase isozyme M; muscle-specific phosphoglycerate mutase

NCBI Summary for PGAM2

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]
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UniProt Comments for PGAM2

PGAM2: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC 5.4.2.11; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.4

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: nucleus; cytosol

Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; cofactor binding; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity

Biological Process: response to mercury ion; striated muscle contraction; glycolysis; dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; spermatogenesis; gluconeogenesis

Disease: Glycogen Storage Disease X
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Research Articles on PGAM2

1. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PGAM2 antibody

 Reference Product  PubMed Publications
 GPI antibody  >3 publications with PGAM2 and GPI
 ALDOA antibody  >1 publications with PGAM2 and ALDOA
 BPGM antibody  >1 publications with PGAM2 and BPGM
 ENO1 antibody  >1 publications with PGAM2 and ENO1
 PGK1 antibody  >1 publications with PGAM2 and PGK1

Pathways associated with anti-PGAM2 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with anti-PGAM2 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >3 publications with PGAM2 and Necrosis
 Disease Models, Animal Antibodies  >1 publications with PGAM2 and Disease Models, Animal
 Abnormalities, Multiple Antibodies  >1 publications with PGAM2 and Abnormalities, Multiple
 Heart Failure Antibodies  >1 publications with PGAM2 and Heart Failure
 Weight Gain Antibodies  >1 publications with PGAM2 and Weight Gain
 Nervous System Diseases Antibodies  >1 publications with PGAM2 and Nervous System Diseases
 Death Antibodies  >1 publications with PGAM2 and Death
 Liver Diseases Antibodies  >1 publications with PGAM2 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with PGAM2 and Kidney Diseases
 Dimauro disease Antibodies  >1 publications with PGAM2 and Dimauro disease

Organs/Tissues associated with anti-PGAM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >14 publications with PGAM2 and Muscle
 Heart Antibodies  >3 publications with PGAM2 and Heart
 Lung Antibodies  >3 publications with PGAM2 and Lung
 Embryonic Tissue Antibodies  >2 publications with PGAM2 and Embryonic Tissue
 Bone Antibodies  >1 publications with PGAM2 and Bone
 Brain Antibodies  >1 publications with PGAM2 and Brain
 Testis Antibodies  >1 publications with PGAM2 and Testis
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