• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SAE1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249048

MBS249048 is an antibody targeting SAE1. Also known as Mouse Monoclonal [clone 1G8] (IgG2b) to Human SAE1. Reactive in Human. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
SAE1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1G8] (IgG2b) to Human SAE1
Product Gene Name
anti-SAE1 antibody
Product Synonym Names
Anti-SAE1 Antibody (clone 1G8) IHC-plus; SAE1; Activator of SUMO1; AOS1; UBLE1A; HSPC140; SUA1; Human SAE1
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1G8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SAE1
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
33,063 Da
OMIM
613294
3D Structure
ModBase 3D Structure for Q9UBE0
Target Species
Human
Immunogen Description
Full length human recombinant protein of human SAE1(NP_005491) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
SAE1 antibody was raised against full length human recombinant protein of human SAE1(NP_005491) produced in HEK293T cell.
Applications Tested/Suitable for anti-SAE1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-SAE1 antibody
IF (1:100), IHC-P (10 ug/ml), WB (1:4000)

Immunohistochemistry (IHC) of anti-SAE1 antibody

Anti-SAE1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-SAE1 Monoclonal Antibody – Cat# MBS249048

Immunofluorescence (IF) of anti-SAE1 antibody

Anti-SAE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE1.
Mouse Anti-SAE1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249048

Western Blot (WB) of anti-SAE1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1.
Mouse Anti-SAE1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249048

Other Notes

Small volumes of anti-SAE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE1. It may not necessarily be applicable to this product.

NCBI GI #
4885585
NCBI GeneID
10055
NCBI Accession #
NP_005491.1
UniProt Primary Accession #
Q9UBE0
UniProt Secondary Accession #
O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6
UniProt Related Accession #
Q9UBE0
GenBank Nucleotide #
NM_005500.2
NCBI Official Full Name
SUMO-activating enzyme subunit 1 isoform a
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names
Ubiquitin-like 1-activating enzyme E1ASUMO-activating enzyme subunit 1, N-terminally processed
Protein Family
SUMO-activating enzyme
UniProt Gene Name
SAE1
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A
UniProt Entry Name
SAE1_HUMAN
NCBI Protein Information
SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme

NCBI Summary for SAE1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
Show more

UniProt Comments for SAE1

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus; cytosol

Molecular Function: protein C-terminus binding; ATP-dependent protein binding; SUMO activating enzyme activity; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification
Show more

Research Articles on SAE1

1. Data indicate the role of anti-SUMO activating enzyme SAE1 and SAE2 antibody as marker of dermatomyositis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SAE1 antibody

 Reference Product  PubMed Publications
 SUMO1 antibody  >26 publications with SAE1 and SUMO1
 UBA2 antibody  >21 publications with SAE1 and UBA2
 RANGAP1 antibody  >6 publications with SAE1 and RANGAP1
 RANBP2 antibody  >4 publications with SAE1 and RANBP2
 SUMO2 antibody  >4 publications with SAE1 and SUMO2
 UBE2I antibody  >2 publications with SAE1 and UBE2I
 SUMO3 antibody  >2 publications with SAE1 and SUMO3
 PCNA antibody  >1 publications with SAE1 and PCNA

Pathways associated with anti-SAE1 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Processing And Activation Of SUMO Pathway antibodies  Processing And Activation Of SUMO Pathway Diagram
 SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway antibodies  SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway Diagram
 SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway antibodies  SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway Diagram
 SUMOylation Pathway antibodies  SUMOylation Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-SAE1 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >2 publications with SAE1 and Mental Disorders
 Disease Models, Animal Antibodies  >2 publications with SAE1 and Disease Models, Animal
 Autoimmune Diseases Antibodies  >1 publications with SAE1 and Autoimmune Diseases
 Abnormalities, Multiple Antibodies  >1 publications with SAE1 and Abnormalities, Multiple
 Muscular Diseases Antibodies  >1 publications with SAE1 and Muscular Diseases
 Brain Diseases Antibodies  >1 publications with SAE1 and Brain Diseases
 Death Antibodies  >1 publications with SAE1 and Death

Organs/Tissues associated with anti-SAE1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with SAE1 and Brain
 Muscle Antibodies  >2 publications with SAE1 and Muscle
 Blood Antibodies  >2 publications with SAE1 and Blood
 Skin Antibodies  >2 publications with SAE1 and Skin
 Vascular Antibodies  >1 publications with SAE1 and Vascular
 Connective Tissue Antibodies  >1 publications with SAE1 and Connective Tissue
 Kidney Antibodies  >1 publications with SAE1 and Kidney
 Uterus Antibodies  >1 publications with SAE1 and Uterus
 Embryonic Tissue Antibodies  >1 publications with SAE1 and Embryonic Tissue
 Lung Antibodies  >1 publications with SAE1 and Lung
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000