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IMP-3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249059

MBS249059 is an antibody targeting IMP-3. Also known as Mouse Monoclonal [clone 8F11] (IgG1) to Human IMP-3 / IGF2BP3. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IMP-3 / IGF2BP3, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 8F11] (IgG1) to Human IMP-3 / IGF2BP3
Product Gene Name
Product Synonym Gene Name
IGF2BP3
Product Synonym Names
Anti-IMP-3 / IGF2BP3 Antibody (clone 8F11) IHC-plus; IGF2BP3; CT98; IGF II mRNA binding protein 3; IMP-3; KOC1; IGF-II mRNA-binding protein 3; IGF2 mRNA-binding protein 3; VICKZ family member 3; Cancer/testis antigen 98; HKOC; VICKZ3; Human IMP-3; IGF2BP3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8F11
Host
Mouse
Species Reactivity
Human
Form/Format
Ascites, 0.03% sodium azide
Purity/Purification
Ascites
Specificity
Human IMP-3 / IGF2BP3
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
21,630 Da
OMIM
608259
3D Structure
ModBase 3D Structure for O00425
Target Species
Human
Immunogen Description
Purified recombinant fragment of human IGF2BP3 expressed in E Coli.
Immunogen Type
Recombinant protein
Immunogen
IMP-3 / IGF2BP3 antibody was raised against purified recombinant fragment of human IGF2BP3 expressed in E Coli.
Applications Tested/Suitable for anti-IMP-3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-IMP-3 antibody
ELISA (1:10000), IHC-P (1:200), WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-IMP-3 antibody

Anti-IMP-3 / IGF2BP3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-IMP-3 Monoclonal Antibody – Cat# MBS249059

Western Blot (WB) of anti-IMP-3 antibody

Western blot of IGF2BP3 mouse mAb against Jurkat (1), K562 (2) and NTERA-2 (3) cell lysate.
Mouse Anti-IMP-3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249059

Other Notes

Small volumes of anti-IMP-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IMP-3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
insulin-like growth factor 2 mRNA-binding protein 3
NCBI Official Synonym Full Names
insulin-like growth factor 2 mRNA binding protein 3
NCBI Official Symbol
IGF2BP3
NCBI Official Synonym Symbols
KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
UniProt Protein Name
Insulin-like growth factor 2 mRNA-binding protein 3
UniProt Synonym Protein Names
IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; hKOC; VICKZ family member 3
UniProt Gene Name
IGF2BP3
UniProt Synonym Gene Names
IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC
UniProt Entry Name
IF2B3_HUMAN
NCBI Protein Information
insulin-like growth factor 2 mRNA-binding protein 3; VICKZ family member 3; cancer/testis antigen 98; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; KH domain containing protein overexpressed in cancer

NCBI Summary for IMP-3

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]
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UniProt Comments for IMP-3

IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 7p11

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; translation; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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