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TUBA1B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249064

MBS249064 is an antibody targeting TUBA1B. Also known as Mouse Monoclonal [clone 17H11.F10] (IgG) to Human TUBA1B. Reactive in Bovine, Chicken, Human, Mouse, Rat. Host: Mouse. More details

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Product Overview

Product Name
TUBA1B, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 17H11.F10] (IgG) to Human TUBA1B
Product Gene Name
anti-TUBA1B antibody
Product Synonym Names
Anti-TUBA1B Antibody (C-Terminus, clone 17H11.F10) IHC-plus; TUBA1B; Alpha-tubulin ubiquitous; Alpha tubulin; K-ALPHA-1; Tubulin; alpha 1b; Tubulin alpha-ubiquitous chain; Tubulin; alpha; ubiquitous; Tubulin K-alpha-1; Tubulin alpha chain; Tubulin alpha-1B chain; Human TUBA1B
Clonality
Monoclonal
Isotype
IgG
Clone Number
17H11.F10
Host
Mouse
Species Reactivity
Bovine, Chicken, Human, Mouse, Rat
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
37,218 Da
OMIM
602530
3D Structure
ModBase 3D Structure for P68363
Target Species
Human
Immunogen Description
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Immunogen Type
Synthetic peptide
Immunogen
TUBA1B antibody was raised against anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-TUBA1B antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TUBA1B antibody
ELISA (1:300000), IF (0.1 ug/ml), IHC-P (5 ug/ml), WB (1:1000)
Usage: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Immunohistochemistry (IHC) of anti-TUBA1B antibody

Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-TUBA1B Monoclonal Antibody – Cat# MBS249064

Immunohistochemistry (IHC) of anti-TUBA1B antibody

Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.
Mouse Anti-TUBA1B Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249064

Immunofluorescence (IF) of anti-TUBA1B antibody

Anti-tubulin Monoclonal antibody-Immunofluorescence. mouse monoclonal anti-tubulin antibody was used with ATTO 425 Goat anti-mouse (shown in red) to detect tubulin by immunofluorescence. Dylight 488 Goat anti-Rabbit (shown in green) was used in the same experiment to detect rabbit anti-Clathrin polyclonal antibody Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.
Mouse Anti-TUBA1B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249064

Immunofluorescence (IF) of anti-TUBA1B antibody

Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) Antibody in red.
Mouse Anti-TUBA1B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249064

Immunofluorescence (IF) of anti-TUBA1B antibody

Immunofluorescence Microscopy - alpha-Tubulin Monoclonal Antibody -. a-tubulin monoclonal antibody detects tubulin (colored RED) in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-a-tubulin and detected with 0.2 ug/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT] detected with DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.
Mouse Anti-TUBA1B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249064

Immunofluorescence (IF) of anti-TUBA1B antibody

Anti-alpha-Tubulin Monoclonal Antibody - Immunofluorescence Microscopy. anti-a-tubulin monoclonal antibody was used at a 0.1 ug/mL to detect tubulin in 4% paraformaldehyde fixed A459 cells. Staining is shown using conventional confocal microscopy (left panel) and by high resolution TCS STED nanoscopy (right panel). DyLight488 conjugated anti-mouse IgG secondary antibody (p/n was used for detection at 1 ug/mL. Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.
Mouse Anti-TUBA1B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249064

Immunofluorescence (IF) of anti-TUBA1B antibody

Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image one showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green. Immunofluorescence image two showing MCF-7 cell staining of Anti-Gli-3 (RABBIT) Antibody in red. Immunofluorescence image three showing MCF-7 cell superimposed staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) antibody in red.
Mouse Anti-TUBA1B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249064

Western Blot (WB) of anti-TUBA1B antibody

Anti-alpha-Tubulin Monoclonal Antibody - Western Blot. HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each. The blot was blocked with Blocking Buffer (MB-070) for 30 min at RT, then washed and incubated with anti-Tubulin monoclonal antibody diluted in Blocking Buffer (p/n MB-070) at 1:1000 for 1 h at RT. After washing, blot was incubated with a 1:40000 dilution of HRP Rb a-Ms IgG secondary antibody in Blocking Buffer (p/n MB-070) for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc 4000 MP.
Mouse Anti-TUBA1B Monoclonal Antibody, Western Blot (WB) – Cat# MBS249064

Other Notes

Small volumes of anti-TUBA1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBA1B. It may not necessarily be applicable to this product.

NCBI GI #
57013276
NCBI GeneID
10376
NCBI Accession #
NP_006073.2
UniProt Primary Accession #
P68363
UniProt Secondary Accession #
P04687; P05209; Q27I68; Q8WU19
UniProt Related Accession #
P68363
GenBank Nucleotide #
NM_006082.2
NCBI Official Full Name
tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin, alpha 1b
NCBI Official Symbol
TUBA1B
NCBI Official Synonym Symbols
K-ALPHA-1
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Synonym Protein Names
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
UniProt Gene Name
TUBA1B
UniProt Entry Name
TBA1B_HUMAN
NCBI Protein Information
tubulin alpha-1B chain; alpha tubulin; tubulin K-alpha-1; alpha-tubulin ubiquitous; tubulin, alpha, ubiquitous; tubulin alpha-ubiquitous chain

UniProt Comments for TUBA1B

TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; cytoplasmic microtubule

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process
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Research Articles on TUBA1B

1. Data indicate that the derivatives exhibited much better potency to inhibit tubulin polymerization but a decreased activity to inhibit Hsp27 chaperone function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TUBA1B antibody

 Products by Pathway  Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway antibodies  Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-TUBA1B antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with TUBA1B and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with TUBA1B and Liver Diseases
 Optic Nerve Diseases Antibodies  >1 publications with TUBA1B and Optic Nerve Diseases
 Brain Diseases Antibodies  >1 publications with TUBA1B and Brain Diseases

Organs/Tissues associated with anti-TUBA1B antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >3 publications with TUBA1B and Eye
 Liver Antibodies  >2 publications with TUBA1B and Liver
 Brain Antibodies  >2 publications with TUBA1B and Brain
 Nerve Antibodies  >1 publications with TUBA1B and Nerve
 Heart Antibodies  >1 publications with TUBA1B and Heart
 Ovary Antibodies  >1 publications with TUBA1B and Ovary
 Testis Antibodies  >1 publications with TUBA1B and Testis
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