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PAICS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249092

MBS249092 is an antibody targeting PAICS. Also known as Mouse Monoclonal [clone 1B4] (IgG2b) to Human PAICS / ADE2. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PAICS / ADE2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1B4] (IgG2b) to Human PAICS / ADE2
Product Gene Name
Product Synonym Gene Name
ADE2
Product Synonym Names
Anti-PAICS / ADE2 Antibody (clone 1B4) IHC-plus; PAICS; ADE2; ADE2H1; AIR carboxylase; AIRC; Multifunctional protein ADE2H1; PAIS; Multifunctional protein ADE2; SAICAR synthetase; Human PAICS; ADE2
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1B4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human PAICS
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
47,958 Da
OMIM
172439
3D Structure
ModBase 3D Structure for P22234
Target Species
Human
Immunogen Description
Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
PAICS / ADE2 antibody was raised against full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.
Applications Tested/Suitable for anti-PAICS antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PAICS antibody
Flo (1:100), IF (1:100), IHC-P (10 ug/ml), WB (1:2000)
Usage: Concentration: 1.00 mg/ml (Lot dependent)

Immunohistochemistry (IHC) of anti-PAICS antibody

Anti-PAICS / ADE2 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-PAICS Monoclonal Antibody – Cat# MBS249092

Immunofluorescence (IF) of anti-PAICS antibody

Anti-PAICS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS.
Mouse Anti-PAICS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249092

Flow Cytometry (FC/FACS) of anti-PAICS antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody, and then analyzed by flow cytometry.
Mouse Anti-PAICS Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249092

Flow Cytometry (FC/FACS) of anti-PAICS antibody

Flow cytometry of Jurkat cells, using anti-PAICS antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Mouse Anti-PAICS Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249092

Western Blot (WB) of anti-PAICS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.
Mouse Anti-PAICS Monoclonal Antibody, Western Blot (WB) – Cat# MBS249092

Other Notes

Small volumes of anti-PAICS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PAICS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
multifunctional protein ADE2 isoform 2
NCBI Official Synonym Full Names
phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
NCBI Official Symbol
PAICS
NCBI Official Synonym Symbols
ADE2; AIRC; PAIS; ADE2H1
UniProt Protein Name
Multifunctional protein ADE2
UniProt Synonym Protein Names
SAICAR synthetase
UniProt Gene Name
PAICS
UniProt Synonym Gene Names
ADE2; AIRC; PAIS; AIRC
UniProt Entry Name
PUR6_HUMAN
NCBI Protein Information
multifunctional protein ADE2; AIR carboxylase; SAICAR synthetase; multifunctional protein ADE2H1

NCBI Summary for PAICS

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for PAICS

PAICS: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.6; Nucleotide Metabolism - purine; EC 4.1.1.21; Lyase

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: membrane; cytosol

Molecular Function: phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; identical protein binding; protein binding; phosphoribosylaminoimidazole carboxylase activity; ATP binding

Biological Process: purine ribonucleoside monophosphate biosynthetic process; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; 'de novo' IMP biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PAICS antibody

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