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MAC-2-BP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249101

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
MAC-2-BP / LGALS3BP, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2B1] (IgG1) to Human MAC-2-BP / LGALS3BP
Product Gene Name
Product Synonym Gene Name
LGALS3BP
Product Synonym Names
Anti-MAC-2-BP / LGALS3BP Antibody (clone 2B1) IHC-plus; LGALS3BP; 90K; Galectin-3-binding protein; Mac-2 BP; Mac-2-binding protein; MAC2BP; L3 antigen; Serum protein 90K; TANGO10B; BTBD17B; M2BP; MAC-2-BP; Tumor-associated antigen 90K; Human MAC-2-BP; LGALS3BP
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
0.81 mg/ml (lot specific)
Specificity
Human MAC-2-BP / LGALS3BP
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
65,331 Da
OMIM
600626
3D Structure
ModBase 3D Structure for Q08380
Target Species
Human
Immunogen Description
Human recombinant protein fragment corresponding to amino acids19-300 of human LGALS3BP(NP_005558) produced in E. coil.
Immunogen Type
Recombinant protein
Immunogen
MAC-2-BP / LGALS3BP antibody was raised against human recombinant protein fragment corresponding to amino acids19-300 of human LGALS3BP(NP_005558) produced in E. coil.
Applications Tested/Suitable for anti-MAC-2-BP antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-MAC-2-BP antibody
Flo (1:100), IHC-P (10 ug/ml), WB (1:2000)

Immunohistochemistry (IHC) of anti-MAC-2-BP antibody

Anti-MAC-2-BP / LGALS3BP antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-MAC-2-BP Monoclonal Antibody – Cat# MBS249101

Flow Cytometry (FC/FACS) of anti-MAC-2-BP antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGALS3BP antibody, and then analyzed by flow cytometry.
Mouse Anti-MAC-2-BP Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249101

Western Blot (WB) of anti-MAC-2-BP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.
Mouse Anti-MAC-2-BP Monoclonal Antibody, Western Blot (WB) – Cat# MBS249101

Other Notes

Small volumes of anti-MAC-2-BP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAC-2-BP purchase

MBS249101 is an antibody targeting MAC-2-BP. Also known as Mouse Monoclonal [clone 2B1] (IgG1) to Human MAC-2-BP / LGALS3BP. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAC-2-BP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
galectin-3-binding protein
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 3 binding protein
NCBI Official Symbol
LGALS3BP
NCBI Official Synonym Symbols
90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
UniProt Protein Name
Galectin-3-binding protein
UniProt Synonym Protein Names
Basement membrane autoantigen p105; Lectin galactoside-binding soluble 3-binding protein; Mac-2-binding protein; MAC2BP; Mac-2 BP; Tumor-associated antigen 90K
UniProt Gene Name
LGALS3BP
UniProt Synonym Gene Names
M2BP; MAC2BP; Mac-2 BP
UniProt Entry Name
LG3BP_HUMAN
NCBI Protein Information
galectin-3-binding protein; MAC2BP; mac-2 BP; L3 antigen; serum protein 90K; Mac-2-binding protein; tumor-associated antigen 90K; basement membrane autoantigen p105; transport and golgi organization 10 homolog B; lectin galactoside-binding soluble 3-binding protein

NCBI Summary for MAC-2-BP

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAC-2-BP

LGALS3BP: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; membrane

Molecular Function: scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cellular defense response; signal transduction; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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