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DPP9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249107

MBS249107 is an antibody targeting DPP9. Also known as Mouse Monoclonal [clone 6A12] (IgG2a) to Human DPP9. Reactive in Dog, Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DPP9, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 6A12] (IgG2a) to Human DPP9
Product Gene Name
Product Synonym Names
Anti-DPP9 Antibody (clone 6A12) IHC-plus; DPP9; Dipeptidyl-peptidase 9; DPRP-2; Dipeptidyl peptidase IX; DP9; DPP IX; DPLP9; Dipeptidyl peptidase 9; Dipeptidylpeptidase 9; DPRP2; Human DPP9
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6A12
Host
Mouse
Species Reactivity
Dog, Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
0.86 mg/ml (lot specific)
Specificity
Human DPP9
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
95,013 Da
OMIM
608258
3D Structure
ModBase 3D Structure for Q86TI2
Target Species
Human
Immunogen Description
Full length human recombinant protein of human DPP9(NP_631898) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
DPP9 antibody was raised against full length human recombinant protein of human DPP9(NP_631898) produced in HEK293T cell.
Applications Tested/Suitable for anti-DPP9 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-DPP9 antibody
Flo (1:100), IHC-P (10 ug/ml), WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-DPP9 antibody

Anti-DPP9 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-DPP9 Monoclonal Antibody – Cat# MBS249107

Flow Cytometry (FC/FACS) of anti-DPP9 antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP9 antibody, and then analyzed by flow cytometry.
Mouse Anti-DPP9 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249107

Flow Cytometry (FC/FACS) of anti-DPP9 antibody

Flow cytometry of Jurkat cells, using anti-DPP9 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-DPP9 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249107

Western Blot (WB) of anti-DPP9 antibody

Western blot of extracts (35 ug) from 9 different cell lines by using anti-DPP9 monoclonal antibody.
Mouse Anti-DPP9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249107

Western Blot (WB) of anti-DPP9 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9.
Mouse Anti-DPP9 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249107

Other Notes

Small volumes of anti-DPP9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DPP9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dipeptidyl peptidase 9
NCBI Official Synonym Full Names
dipeptidyl-peptidase 9
NCBI Official Symbol
DPP9
NCBI Official Synonym Symbols
DP9; DPLP9; DPRP2; DPRP-2
UniProt Protein Name
Dipeptidyl peptidase 9
UniProt Synonym Protein Names
Dipeptidyl peptidase IV-related protein 2; DPRP-2; Dipeptidyl peptidase IX; DPP IX; Dipeptidyl peptidase-like protein 9; DPLP9
Protein Family
UniProt Gene Name
DPP9
UniProt Synonym Gene Names
DPRP2; DP9; DPRP-2; DPP IX; DPLP9
UniProt Entry Name
DPP9_HUMAN
NCBI Protein Information
dipeptidyl peptidase 9; DPP IX; dipeptidylpeptidase 9; dipeptidyl peptidase IX; dipeptidyl peptidase-like protein 9; dipeptidyl peptidase IV-related protein 2; dipeptidyl peptidase IV-related protein-2

NCBI Summary for DPP9

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for DPP9

DPP9: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Belongs to the peptidase S9B family. DPPIV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.14.5; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; serine-type peptidase activity; aminopeptidase activity

Biological Process: proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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