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PPID Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249136

MBS249136 is an antibody targeting PPID. Also known as Mouse Monoclonal [clone 4C7] (IgG2a,k) to Human PPID / Cyclophilin D. Reactive in Human. Host: Mouse. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
PPID / Cyclophilin D, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4C7] (IgG2a,k) to Human PPID / Cyclophilin D
Product Gene Name
anti-PPID antibody
Product Synonym Names
Anti-PPID / Cyclophilin D Antibody (clone 4C7) IHC-plus; Cyclophilin-40; CYP40; CYP-40; Cyclophilin 40; Cyclophilin-related protein; CYPD; PPIase D; Cyclophilin D; Peptidylprolyl isomerase D; Rotamase D; Human PPID; Cyclophilin D
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4C7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PPID / Cyclophilin D
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
Molecular Weight
40,764 Da
OMIM
601753
3D Structure
ModBase 3D Structure for Q08752
Target Species
Human
Immunogen Description
PPID (NP_005029.1, aa1-370 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
GST fusion protein
Immunogen
PPID / Cyclophilin D antibody was raised against pPID (NP_005029.1, aa1-370 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-PPID antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PPID antibody
ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB
Usage: Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein. Immunohistochemistry: Paraffin.

Immunohistochemistry (IHC) of anti-PPID antibody

Anti-PPID / Cyclophilin D antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-PPID Monoclonal Antibody – Cat# MBS249136

Immunohistochemistry (IHC) of anti-PPID antibody

Anti-PPID / Cyclophilin D antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-PPID Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249136

Immunofluorescence (IF) of anti-PPID antibody

Immunofluorescence of monoclonal antibody to PPID on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PPID Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249136

Western Blot (WB) of anti-PPID antibody

Western Blot analysis of PPID expression in transfected 293T cell line by PPID monoclonal antibody (M01), clone 4C7.Lane 1: PPID transfected lysate (Predicted MW: 40.8 KDa).Lane 2: Non-transfected lysate.
Mouse Anti-PPID Monoclonal Antibody, Western Blot (WB) – Cat# MBS249136

ELISA (EIA) of anti-PPID antibody

Detection limit for recombinant GST tagged PPID is 3 ng/ml as a capture antibody.
Mouse Anti-PPID Monoclonal Antibody, ELISA (EIA) – Cat# MBS249136

Other Notes

Small volumes of anti-PPID antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPID. It may not necessarily be applicable to this product.

NCBI GI #
4826932
NCBI GeneID
5481
NCBI Accession #
NP_005029.1
UniProt Primary Accession #
Q08752
UniProt Secondary Accession #
B2R9V2
UniProt Related Accession #
Q08752
GenBank Nucleotide #
NM_005038.2
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase D
NCBI Official Synonym Full Names
peptidylprolyl isomerase D
NCBI Official Symbol
PPID
NCBI Official Synonym Symbols
CYPD; CYP-40
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase D
UniProt Synonym Protein Names
40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D
Protein Family
Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name
PPID
UniProt Synonym Gene Names
CYP40; CYPD; PPIase D; CYP-40
UniProt Entry Name
PPID_HUMAN
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase D; PPIase D; rotamase D; cyclophilin D; cyclophilin 40; cyclophilin-40; cyclophilin-related protein; 40 kDa peptidyl-prolyl cis-trans isomerase D

NCBI Summary for PPID

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPID

PPID: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.

Protein type: Isomerase; EC 5.2.1.8; Nucleolus

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; heat shock protein binding; estrogen receptor binding; Hsp70 protein binding; Hsp90 protein binding; cyclosporin A binding; transcription factor binding

Biological Process: release of virus from host; protein transport; positive regulation of viral genome replication; protein peptidyl-prolyl isomerization; positive regulation of protein secretion; protein folding; positive regulation of apoptosis; apoptosis; protein complex assembly; negative regulation of transcription from RNA polymerase II promoter
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Research Articles on PPID

1. The Photorhabdus toxins TccC3 and TccC5 interacted with Hsp90, FKBP51, Cyp40 and CypA, suggesting a role of these host cell factors in translocation and/or refolding of the ADP-ribosyltransferases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PPID antibody

 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram

Diseases associated with anti-PPID antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >6 publications with PPID and Disease Models, Animal
 Necrosis Antibodies  >5 publications with PPID and Necrosis
 Hyperplasia Antibodies  >4 publications with PPID and Hyperplasia
 Inflammation Antibodies  >3 publications with PPID and Inflammation
 Hypertrophy Antibodies  >2 publications with PPID and Hypertrophy
 Fibrosis Antibodies  >2 publications with PPID and Fibrosis
 Weight Loss Antibodies  >2 publications with PPID and Weight Loss
 Poisoning Antibodies  >2 publications with PPID and Poisoning
 Cardiovascular Diseases Antibodies  >2 publications with PPID and Cardiovascular Diseases
 Drug-Induced Liver Injury Antibodies  >2 publications with PPID and Drug-Induced Liver Injury

Organs/Tissues associated with anti-PPID antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >38 publications with PPID and Brain
 Blood Antibodies  >35 publications with PPID and Blood
 Heart Antibodies  >3 publications with PPID and Heart
 Liver Antibodies  >3 publications with PPID and Liver
 Lung Antibodies  >2 publications with PPID and Lung
 Prostate Antibodies  >2 publications with PPID and Prostate
 Thyroid Antibodies  >1 publications with PPID and Thyroid
 Pancreas Antibodies  >1 publications with PPID and Pancreas
 Skin Antibodies  >1 publications with PPID and Skin
 Intestine Antibodies  >1 publications with PPID and Intestine
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