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HIST1H3D Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249138

MBS249138 is an antibody targeting HIST1H3D. Also known as Mouse Monoclonal [clone 1D8] (IgG3,k) to Human HIST1H3D. Reactive in Human, Mouse. Host: Mouse. More details

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Product Overview

Product Name
HIST1H3D, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1D8] (IgG3,k) to Human HIST1H3D
Product Gene Name
anti-HIST1H3D antibody
Product Synonym Names
Anti-HIST1H3D Antibody (clone 1D8) IHC-plus; HIST1H3D; Histone 1; H3d; Histone cluster 1; H3d; Histone H3.1; Human HIST1H3D
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
1D8
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
PBS, pH 7.4.
Purity/Purification
Protein A Purified
Concentration
0.31 mg/ml (lot specific)
Specificity
Human HIST1H3H
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
15,404 Da
OMIM
gene 602819
Target Species
Human
Immunogen Description
HIST1H3D (NP_003521, 1 a.a. ~ 61 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Applications Tested/Suitable for anti-HIST1H3D antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-HIST1H3D antibody
ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Cell lysate, Cell lysate, Cell lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-HIST1H3D antibody

Anti-HIST1H3D antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-HIST1H3D Monoclonal Antibody – Cat# MBS249138

Immunofluorescence (IF) of anti-HIST1H3D antibody

Immunofluorescence of monoclonal antibody to HIST1H3D on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-HIST1H3D Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249138

Western Blot (WB) of anti-HIST1H3D antibody

HIST1H3D monoclonal antibody (M01), clone 1D8 Western blot of HIST1H3D expression in HeLa NE.
Mouse Anti-HIST1H3D Monoclonal Antibody, Western Blot (WB) – Cat# MBS249138

Western Blot (WB) of anti-HIST1H3D antibody

HIST1H3D monoclonal antibody (M01), clone 1D8. Western blot of HIST1H3D expression in NIH/3T3.
Mouse Anti-HIST1H3D Monoclonal Antibody, Western Blot (WB) – Cat# MBS249138

Western Blot (WB) of anti-HIST1H3D antibody

HIST1H3D monoclonal antibody (M01), clone 1D8. Western blot of HIST1H3D expression in Raw 264.7.
Mouse Anti-HIST1H3D Monoclonal Antibody, Western Blot (WB) – Cat# MBS249138

Other Notes

Small volumes of anti-HIST1H3D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H3D. It may not necessarily be applicable to this product.

NCBI GI #
21071021
NCBI GeneID
8351
NCBI Accession #
NP_003521.2
UniProt Secondary Accession #
P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
UniProt Related Accession #
P68431
GenBank Nucleotide #
NM_003530.4
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3d
NCBI Official Symbol
HIST1H3D
NCBI Official Synonym Symbols
H3/b; H3FB
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
NCBI Protein Information
histone H3.1; histone H3/b; histone 1, H3d; H3 histone family, member B

NCBI Summary for HIST1H3D

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Jul 2008]
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UniProt Comments for HIST1H3D

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine
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Research Articles on HIST1H3D

1. HIST1H3D expression in the bone marrow, peripheral blood, and primary tumor of stage IV patients is higher than that in stage I patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HIST1H3D antibody

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 Condensation Of Prophase Chromosomes Pathway antibodies  Condensation Of Prophase Chromosomes Pathway Diagram

Diseases associated with anti-HIST1H3D antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1 publications with HIST1H3D and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >1 publications with HIST1H3D and Neoplasms, Experimental
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