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SSB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249153

MBS249153 is an antibody targeting SSB. Also known as Mouse Monoclonal [clone 1E11] (IgG2a) to Human SSB / La. Reactive in Dog, Human, Monkey, Mouse, Rat. Host: Mouse. More details

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Product Overview

Product Name
SSB / La, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1E11] (IgG2a) to Human SSB / La
Product Gene Name
anti-SSB antibody
Product Synonym Gene Name
La
Product Synonym Names
Anti-SSB / La Antibody (clone 1E11) IHC-plus; SSB; Autoantigen La; La; La ribonucleoprotein; Lupus La antigen; SS-B; SS-B/La protein; La autoantigen; LARP3; Lupus La protein; Human SSB; La
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1E11
Host
Mouse
Species Reactivity
Dog, Human, Monkey, Mouse, Rat
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SSB / La
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
46,837 Da
OMIM
109090
3D Structure
ModBase 3D Structure for P05455
Target Species
Human
Immunogen Description
Protein expressed in 293T cell transfected with human SSB expression vector.
Immunogen Type
Transfected Cells
Immunogen
SSB / La antibody was raised against protein expressed in 293T cell transfected with human SSB expression vector.
Applications Tested/Suitable for anti-SSB antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-SSB antibody
Flo (1:100), IF (1:50 - 1:100), IHC-P (10 ug/ml), IP, WB (1:1000 - 1:2000)

Immunohistochemistry (IHC) of anti-SSB antibody

Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-SSB Monoclonal Antibody – Cat# MBS249153

Immunofluorescence (IF) of anti-SSB antibody

Anti-SSB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
Mouse Anti-SSB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249153

Immunofluorescence (IF) of anti-SSB antibody

Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody.
Mouse Anti-SSB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249153

Flow Cytometry (FC/FACS) of anti-SSB antibody

Flow cytometry of Jurkat cells, using anti-SSB antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-SSB Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249153

Western Blot (WB) of anti-SSB antibody

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Mouse Anti-SSB Monoclonal Antibody, Western Blot (WB) – Cat# MBS249153

Western Blot (WB) of anti-SSB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Mouse Anti-SSB Monoclonal Antibody, Western Blot (WB) – Cat# MBS249153

Immunoprecipitation (IP) of anti-SSB antibody

Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Mouse Anti-SSB Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS249153

Other Notes

Small volumes of anti-SSB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SSB. It may not necessarily be applicable to this product.

NCBI GI #
10835067
NCBI GeneID
6741
NCBI Accession #
NP_003133.1
UniProt Primary Accession #
P05455
UniProt Secondary Accession #
Q15367; Q53XJ4
UniProt Related Accession #
P05455
GenBank Nucleotide #
NM_003142.4
NCBI Official Full Name
lupus La protein
NCBI Official Synonym Full Names
Sjogren syndrome antigen B (autoantigen La)
NCBI Official Symbol
SSB
NCBI Official Synonym Symbols
La; LARP3; La/SSB
UniProt Protein Name
Lupus La protein
UniProt Synonym Protein Names
La autoantigen; La ribonucleoprotein; Sjoegren syndrome type B antigen; SS-B
Protein Family
Heat shock protein
UniProt Gene Name
SSB
UniProt Synonym Gene Names
SS-B
UniProt Entry Name
LA_HUMAN
NCBI Protein Information
lupus La protein; SS-B; autoantigen La; la autoantigen; SS-B/La protein; lupus La antigen; sjoegren syndrome type B antigen; La ribonucleoprotein domain family, member 3

NCBI Summary for SSB

The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]
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UniProt Comments for SSB

SSB: a nuclear protein that plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5s, 7s, and 7-2 RNAs.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: nucleus; ribonucleoprotein complex

Molecular Function: mRNA binding; protein binding; nucleotide binding; tRNA binding

Biological Process: RNA processing; histone mRNA metabolic process; tRNA modification
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Research Articles on SSB

1. Primary Sjogren's patients with WSP form a benign subgroup, with a lower prevalence of anti-SSB and extraglandular manifestations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SSB antibody

 Reference Product  PubMed Publications
 NCL antibody  >3 publications with SSB and NCL

Pathways associated with anti-SSB antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Termination Pathway antibodies  RNA Polymerase III Transcription Termination Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram

Diseases associated with anti-SSB antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >250 publications with SSB and Cardiovascular Diseases
 Heart Diseases Antibodies  >193 publications with SSB and Heart Diseases
 Vascular Diseases Antibodies  >88 publications with SSB and Vascular Diseases
 Inflammation Antibodies  >72 publications with SSB and Inflammation
 Lung Diseases Antibodies  >50 publications with SSB and Lung Diseases
 Kidney Diseases Antibodies  >49 publications with SSB and Kidney Diseases
 Necrosis Antibodies  >39 publications with SSB and Necrosis
 Weight Gain Antibodies  >36 publications with SSB and Weight Gain
 Hypertension Antibodies  >31 publications with SSB and Hypertension
 Weight Loss Antibodies  >21 publications with SSB and Weight Loss

Organs/Tissues associated with anti-SSB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >627 publications with SSB and Blood
 Heart Antibodies  >246 publications with SSB and Heart
 Skin Antibodies  >158 publications with SSB and Skin
 Salivary Gland Antibodies  >149 publications with SSB and Salivary Gland
 Liver Antibodies  >136 publications with SSB and Liver
 Connective Tissue Antibodies  >126 publications with SSB and Connective Tissue
 Mouth Antibodies  >112 publications with SSB and Mouth
 Lung Antibodies  >94 publications with SSB and Lung
 Muscle Antibodies  >84 publications with SSB and Muscle
 Brain Antibodies  >71 publications with SSB and Brain
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