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DIXDC1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249156

MBS249156 is an antibody targeting DIXDC1. Also known as Mouse Monoclonal [clone 6B6] (IgG1) to Human DIXDC1. Reactive in Human, Monkey. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DIXDC1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 6B6] (IgG1) to Human DIXDC1
Product Gene Name
Product Synonym Names
Anti-DIXDC1 Antibody (clone 6B6) IHC-plus; DIXDC1; Coiled-coil-DIX1; CCD1; DIX domain containing 1; Coiled-coil protein DIX1; KIAA1735; Dixin; Human DIXDC1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6B6
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human DIXDC1
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Target Species
Human
Immunogen Description
Full length human recombinant protein of human DIXDC1(NP_219493) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
DIXDC1 antibody was raised against full length human recombinant protein of human DIXDC1(NP_219493) produced in HEK293T cell.
Applications Tested/Suitable for anti-DIXDC1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-DIXDC1 antibody
IF (1:100), IHC-P (15 ug/ml), WB (1:200 - 1:2000)

Immunohistochemistry (IHC) of anti-DIXDC1 antibody

Anti-DIXDC1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Mouse Anti-DIXDC1 Monoclonal Antibody – Cat# MBS249156

Immunofluorescence (IF) of anti-DIXDC1 antibody

Anti-DIXDC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DIXDC1.
Mouse Anti-DIXDC1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249156

Western Blot (WB) of anti-DIXDC1 antibody

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-DIXDC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Mouse Anti-DIXDC1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249156

Western Blot (WB) of anti-DIXDC1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DIXDC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DIXDC1.
Mouse Anti-DIXDC1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249156

Other Notes

Small volumes of anti-DIXDC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DIXDC1. It may not necessarily be applicable to this product.

NCBI GeneID
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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