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NRBP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249178

NRBP contains a kinase-homology domain and exhibits an associated kinase activity. In addition, NRBP is a multidomain putative adapter protein containing two putative nuclear receptor binding motifs, a putative binding domain for Src homology-2 domain… More details

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Product Overview

Product Name
NRBP1 / NRBP, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2C8] (IgG2a) to Human NRBP1 / NRBP
Product Gene Name
anti-NRBP1 antibody
Product Synonym Gene Name
NRBP
Product Synonym Names
Anti-NRBP1 / NRBP Antibody (clone 2C8) IHC-plus; NRBP1; MUDPNP; NRBP; BCON3; MADM; Human NRBP1; NRBP
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2C8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human NRBP1 / MADM
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
59,845 Da
OMIM
606010
3D Structure
ModBase 3D Structure for Q9UHY1
Target Species
Human
Immunogen Description
Protein expressed in 293T cell transfected with human NRBP1 expression vector.
Immunogen Type
Transfected Cells
Immunogen
NRBP1 / NRBP antibody was raised against protein expressed in 293T cell transfected with human NRBP1 expression vector.

Product Categories/Family for anti-NRBP1 antibody

Family: Protein Kinase; Subfamily: NRBP
Applications Tested/Suitable for anti-NRBP1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-NRBP1 antibody
Flo (1:100), IHC-P (10 ug/ml), IP, WB (1:500)
Usage: Concentration: 0.5-1.0 mg/ml (Lot dependent)

Immunohistochemistry (IHC) of anti-NRBP1 antibody

Anti-NRBP1 / NRBP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-NRBP1 Monoclonal Antibody – Cat# MBS249178

Flow Cytometry (FC/FACS) of anti-NRBP1 antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody, and then analyzed by flow cytometry.
Mouse Anti-NRBP1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249178

Western Blot (WB) of anti-NRBP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.
Mouse Anti-NRBP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249178

Immunoprecipitation (IP) of anti-NRBP1 antibody

Immunoprecipitation(IP) of NRBP1 by using monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NRBP1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Mouse Anti-NRBP1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS249178

Other Notes

Small volumes of anti-NRBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NRBP1 purchase

MBS249178 is an antibody targeting NRBP1. Also known as Mouse Monoclonal [clone 2C8] (IgG2a) to Human NRBP1 / NRBP. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NRBP1. It may not necessarily be applicable to this product.

NCBI GI #
7019333
NCBI GeneID
29959
NCBI Accession #
NP_037524.1
UniProt Primary Accession #
Q9UHY1
UniProt Secondary Accession #
Q53FZ5; Q96SU3; B3KV40; D6W558
UniProt Related Accession #
Q9UHY1
GenBank Nucleotide #
NM_013392.2
NCBI Official Full Name
nuclear receptor-binding protein
NCBI Official Synonym Full Names
nuclear receptor binding protein 1
NCBI Official Symbol
NRBP1
NCBI Official Synonym Symbols
MADM; NRBP; BCON3; MUDPNP
UniProt Protein Name
Nuclear receptor-binding protein
Protein Family
Nuclear receptor-binding protein
UniProt Gene Name
NRBP1
UniProt Entry Name
NRBP_HUMAN
NCBI Protein Information
nuclear receptor-binding protein; multiple domain putative nuclear protein; myeloid leukemia factor 1 adaptor molecule

UniProt Comments for NRBP1

NRBP1: May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; NRBP family

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; membrane; lamellipodium; perinuclear region of cytoplasm; endomembrane system; cell cortex

Molecular Function: protein binding; protein homodimerization activity; ATP binding; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; ER to Golgi vesicle-mediated transport; gene expression; protein amino acid phosphorylation
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Research Articles on NRBP1

1. Targeting this we performed homology modeling and protein-protein docking study of NS3 with NRBP (Nuclear Receptor Binding Protein) of human as it has been proved that NS3 of DENV interacts with NRBP which causes cellular trafficking in human cell.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NRBP1 antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram

Diseases associated with anti-NRBP1 antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with NRBP1 and Kidney Diseases
 Hyperplasia Antibodies  >1 publications with NRBP1 and Hyperplasia
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