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NMI Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249209

MBS249209 is an antibody targeting NMI. Also known as Mouse Monoclonal [clone 9D8] (IgG2b,k) to Human NMI. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
NMI, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 9D8] (IgG2b,k) to Human NMI
Product Gene Name
Product Synonym Names
Anti-NMI Antibody (clone 9D8) IHC-plus; NMI; Hou; N-myc and STAT interactor; N-myc-interactor; N-myc (and STAT) interactor; N-myc interactor; Human NMI
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
9D8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
Molecular Weight
35,057 Da
OMIM
603525
3D Structure
ModBase 3D Structure for Q13287
Target Species
Human
Immunogen Description
NMI (AAH01268, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
NMI antibody was raised against nMI (AAH01268, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-NMI antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-NMI antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-NMI antibody

Anti-NMI antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-NMI Monoclonal Antibody – Cat# MBS249209

Western Blot (WB) of anti-NMI antibody

Western blot of NMI expression in transfected 293T cell line by NMI monoclonal antibody (M01), clone 9D8.
Mouse Anti-NMI Monoclonal Antibody, Western Blot (WB) – Cat# MBS249209

Western Blot (WB) of anti-NMI antibody

NMI monoclonal antibody (M01), clone 9D8 Western blot of NMI expression in HeLa.
Mouse Anti-NMI Monoclonal Antibody, Western Blot (WB) – Cat# MBS249209

RNA interference of anti-NMI antibody

Western blot of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody, clone 9D8. GAPDH (36.1 kD) used as specificity and l.
Mouse Anti-NMI Monoclonal Antibody, RNA interference – Cat# MBS249209

ELISA (EIA) of anti-NMI antibody

Detection limit for recombinant GST tagged NMI is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-NMI Monoclonal Antibody, ELISA (EIA) – Cat# MBS249209

Other Notes

Small volumes of anti-NMI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NMI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
N-myc-interactor
NCBI Official Synonym Full Names
N-myc (and STAT) interactor
NCBI Official Symbol
NMI
UniProt Protein Name
N-myc-interactor
UniProt Synonym Protein Names
N-myc and STAT interactor
Protein Family
UniProt Gene Name
NMI
UniProt Synonym Gene Names
Nmi
UniProt Entry Name
NMI_HUMAN
NCBI Protein Information
N-myc-interactor; N-myc interactor

NCBI Summary for NMI

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]
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UniProt Comments for NMI

NMI: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. Belongs to the NMI family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; inflammatory response; JAK-STAT cascade
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NMI antibody

 Products by Pathway  Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
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