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GBP2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249259

GBP2 Antibody, GBP-2 Antibody, HuGBP-2 Antibody, Guanylate binding protein 2 Antibody, GTP-binding protein 2 Antibody Description: Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes… More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GBP2, Monoclonal Antibody
Full Product Name
Anti-GBP2 Antibody (clone 5C8) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 5C8] (IgG2b) to Human GBP2; Human GBP2; GBP-2; HuGBP-2; Guanylate binding protein 2; GTP-binding protein 2
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5C8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
1.5 mg/ml (lot specific)
Specificity
Human GBP2
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
67,209 Da
OMIM
600412
3D Structure
ModBase 3D Structure for P32456
Immunogen
GBP2 antibody was raised against full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-GBP2 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-GBP2 antibody
Flow Cytometry (1:100)
IF (1:100)
IHC-P (5 ug/ml)
WB (1:2000)

Immunohistochemistry - Paraffin (IHC) of anti-GBP2 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-GBP2 Monoclonal Antibody – Cat# MBS249259

Immunohistochemistry - Paraffin (IHC) of anti-GBP2 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-GBP2 Monoclonal Antibody, Immunohistochemistry - Paraffin (IHC) – Cat# MBS249259

Immunofluorescence (IF) of anti-GBP2 antibody

Anti-GBP2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2.
Mouse Anti-GBP2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249259

Western Blot (WB) of anti-GBP2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2.
Mouse Anti-GBP2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249259

Flow Cytometry (FC/FACS) of anti-GBP2 antibody

HEK293T cells transfected with either pCMV6-ENTRY GBP2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-GBP2 mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
Mouse Anti-GBP2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249259

Other Notes

Small volumes of anti-GBP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GBP2 purchase

MBS249259 is an antibody targeting GBP2. Also known as Anti-GBP2 Antibody (clone 5C8) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GBP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
guanylate-binding protein 2
NCBI Official Synonym Full Names
guanylate binding protein 2
NCBI Official Symbol
GBP2
UniProt Protein Name
Guanylate-binding protein 2
UniProt Synonym Protein Names
GTP-binding protein 2; GBP-2; HuGBP-2; Guanine nucleotide-binding protein 2; Interferon-induced guanylate-binding protein 2
UniProt Gene Name
GBP2
UniProt Synonym Gene Names
GBP-2; HuGBP-2
UniProt Entry Name
GBP2_HUMAN
NCBI Protein Information
guanylate-binding protein 2

NCBI Summary for GBP2

This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. [provided by RefSeq, Jul 2013]
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UniProt Comments for GBP2

GBP2: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Hydrolase; Vesicle

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: cytosol; Golgi membrane; perinuclear region of cytoplasm

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: cytokine and chemokine mediated signaling pathway; immune response; metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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