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VASP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249264

VASP Antibody Description: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell… More details

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Product Overview

Product Name
VASP, Monoclonal Antibody
Full Product Name
Anti-VASP Antibody (clone 4D6) IHC-plus
Product Gene Name
anti-VASP antibody
Product Synonym Names
Mouse Monoclonal [clone 4D6] (IgG1) to Human VASP; Human VASP; VASP
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4D6
Host
Mouse
Species Reactivity
Rat, Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Affinity purified
Concentration
0.9 mg/ml (lot specific)
Specificity
Human VASP
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
39,830 Da
OMIM
601703
3D Structure
ModBase 3D Structure for P50552
Immunogen
VASP antibody was raised against full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.
Immunogen Description
Full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-VASP antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-VASP antibody
Flow Cytometry (1:100)
IF (1:100)
IHC-P (10 ug/ml)
WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-VASP antibody

Anti-VASP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-VASP Monoclonal Antibody – Cat# MBS249264

Immunofluorescence (IF) of anti-VASP antibody

Anti-VASP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.
Mouse Anti-VASP Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249264

Western Blot (WB) of anti-VASP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.
Mouse Anti-VASP Monoclonal Antibody, Western Blot (WB) – Cat# MBS249264

Western Blot (WB) of anti-VASP antibody

Western blot of extracts (35 ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Mouse Anti-VASP Monoclonal Antibody, Western Blot (WB) – Cat# MBS249264

Flow Cytometry (FC/FACS) of anti-VASP antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VASP antibody, and then analyzed by flow cytometry.
Mouse Anti-VASP Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249264

Flow Cytometry (FC/FACS) of anti-VASP antibody

Flow cytometry of Jurkat cells, using anti-VASP antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-VASP Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249264

Flow Cytometry (FC/FACS) of anti-VASP antibody

Flow cytometry of HeLa cells, using anti-VASP antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-VASP Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249264

Other Notes

Small volumes of anti-VASP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VASP purchase

MBS249264 is an antibody targeting VASP. Also known as Anti-VASP Antibody (clone 4D6) IHC-plus. Reactive in Rat, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VASP. It may not necessarily be applicable to this product.

NCBI GI #
4507869
NCBI GeneID
7408
NCBI Accession #
NP_003361.1
UniProt Primary Accession #
P50552
UniProt Secondary Accession #
Q6PIZ1; Q93035; B2RBT9
UniProt Related Accession #
P50552
GenBank Nucleotide #
NM_003370.3
NCBI Official Full Name
vasodilator-stimulated phosphoprotein
NCBI Official Synonym Full Names
vasodilator-stimulated phosphoprotein
NCBI Official Symbol
VASP
UniProt Protein Name
Vasodilator-stimulated phosphoprotein
Protein Family
Protein
UniProt Gene Name
VASP
UniProt Synonym Gene Names
VASP
UniProt Entry Name
VASP_HUMAN
NCBI Protein Information
vasodilator-stimulated phosphoprotein

NCBI Summary for VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
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UniProt Comments for VASP

VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; filopodium membrane; focal adhesion; plasma membrane; tight junction

Molecular Function: actin binding; profilin binding; protein binding; SH3 domain binding

Biological Process: actin polymerization and/or depolymerization; axon guidance; neural tube closure; positive regulation of actin filament polymerization; protein homotetramerization; T cell receptor signaling pathway
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Research Articles on VASP

1. Data show that tumor necrosis factor-alpha (TNF-alpha) increased A549 lung adenocarcinoma cell permeability by repressing vasodilator-stimulated phosphoprotein (VASP) expression through the activation of hypoxia inducible factor 1 alpha subunit (HIF-1alpha).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VASP antibody

 Reference Product  PubMed Publications
 ZYX antibody  >35 publications with VASP and ZYX
 CDC42 antibody  >28 publications with VASP and CDC42
 WASL antibody  >12 publications with VASP and WASL
 FYB antibody  >9 publications with VASP and FYB
 LPP antibody  >7 publications with VASP and LPP
 PRKG1 antibody  >6 publications with VASP and PRKG1
 APBB1IP antibody  >3 publications with VASP and APBB1IP
 FBLIM1 antibody  >3 publications with VASP and FBLIM1
 PRKG2 antibody  >2 publications with VASP and PRKG2

Pathways associated with anti-VASP antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-extracellular Matrix Interactions Pathway antibodies  Cell-extracellular Matrix Interactions Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 E-cadherin Signaling In Keratinocytes Pathway antibodies  E-cadherin Signaling In Keratinocytes Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-VASP antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >216 publications with VASP and Cardiovascular Diseases
 Thrombosis Antibodies  >81 publications with VASP and Thrombosis
 Myocardial Infarction Antibodies  >48 publications with VASP and Myocardial Infarction
 Disease Models, Animal Antibodies  >45 publications with VASP and Disease Models, Animal
 Nervous System Diseases Antibodies  >43 publications with VASP and Nervous System Diseases
 Inflammation Antibodies  >41 publications with VASP and Inflammation
 Pain Antibodies  >25 publications with VASP and Pain
 Hemorrhage Antibodies  >22 publications with VASP and Hemorrhage
 Hypertension Antibodies  >21 publications with VASP and Hypertension
 Breast Neoplasms Antibodies  >17 publications with VASP and Breast Neoplasms

Organs/Tissues associated with anti-VASP antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >421 publications with VASP and Blood
 Vascular Antibodies  >179 publications with VASP and Vascular
 Muscle Antibodies  >96 publications with VASP and Muscle
 Brain Antibodies  >73 publications with VASP and Brain
 Embryonic Tissue Antibodies  >40 publications with VASP and Embryonic Tissue
 Kidney Antibodies  >36 publications with VASP and Kidney
 Lung Antibodies  >32 publications with VASP and Lung
 Liver Antibodies  >23 publications with VASP and Liver
 Bone Antibodies  >18 publications with VASP and Bone
 Eye Antibodies  >12 publications with VASP and Eye
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