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TCL / RHOJ Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249281

RHOJ Antibody, ARHJ Antibody, Ras homolog family member J Antibody, TC10-like Rho GTPase Antibody, TC10B Antibody, RAS-like, family 7, member B Antibody, RASL7B Antibody, RHOI Antibody, Tc10-like GTP-binding protein Antibody, TCL Antibody Description:… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TCL / RHOJ, Monoclonal Antibody
Full Product Name
Anti-TCL / RHOJ Antibody (clone 1D7) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 1D7] (IgG2a) to Human TCL / RHOJ; Human TCL / RHOJ; ARHJ; Ras homolog family member J; TC10-like Rho GTPase; TC10B; RAS-like; family 7; member B; RASL7B; RHOI; Tc10-like GTP-binding protein
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1D7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
1 mg/ml (lot specific)
Specificity
Human TCL / RHOJ
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
21,255 Da
OMIM
607653
3D Structure
ModBase 3D Structure for Q9H4E5
Immunogen
TCL / RHOJ antibody was raised against full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-TCL / RHOJ antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-TCL / RHOJ antibody
IF (1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Immunohistochemistry (IHC) of anti-TCL / RHOJ antibody

Anti-TCL / RHOJ antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-TCL / RHOJ Monoclonal Antibody – Cat# MBS249281

Immunofluorescence (IF) of anti-TCL / RHOJ antibody

Anti-RHOJ mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ.
Mouse Anti-TCL / RHOJ Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249281

Western Blot (WB) of anti-TCL / RHOJ antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOJ.
Mouse Anti-TCL / RHOJ Monoclonal Antibody, Western Blot (WB) – Cat# MBS249281

Other Notes

Small volumes of anti-TCL / RHOJ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TCL / RHOJ purchase

MBS249281 is an antibody targeting TCL / RHOJ. Also known as Anti-TCL / RHOJ Antibody (clone 1D7) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TCL / RHOJ. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rho-related GTP-binding protein RhoJ
NCBI Official Synonym Full Names
ras homolog family member J
NCBI Official Symbol
RHOJ
NCBI Official Synonym Symbols
TCL; ARHJ; TC10B; RASL7B
UniProt Protein Name
Rho-related GTP-binding protein RhoJ
UniProt Synonym Protein Names
Ras-like protein family member 7B; Tc10-like GTP-binding protein
UniProt Gene Name
RHOJ
UniProt Synonym Gene Names
ARHJ; RASL7B; RHOI; TCL
UniProt Entry Name
RHOJ_HUMAN
NCBI Protein Information
rho-related GTP-binding protein RhoJ

NCBI Summary for TCL / RHOJ

This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]
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UniProt Comments for TCL / RHOJ

RHOJ: GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: cytosol; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TCL / RHOJ antibody

 Reference Product  PubMed Publications
 CDC42 antibody  >12 publications with TCL / RHOJ and CDC42
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