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HHEX / HEX Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249287

HHEX Antibody, HEX Antibody, HMPH Antibody, Homeobox protein HEX Antibody, HOX11L-PEN Antibody, PRHX Antibody, Homeobox protein PRH Antibody, PRH Antibody Description: Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in… More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HHEX / HEX, Monoclonal Antibody
Full Product Name
Anti-HHEX / HEX Antibody (clone 3F4) IHC-plus
Product Gene Name
anti-HHEX / HEX antibody
Product Synonym Names
Mouse Monoclonal [clone 3F4] (IgG2b) to Human HHEX / HEX; Human HHEX / HEX; HMPH; Homeobox protein HEX; HOX11L-PEN; PRHX; Homeobox protein PRH; PRH
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
0.5-1.0 mg/ml (lot specific)
Specificity
Human HHEX / HEX
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
3D Structure
ModBase 3D Structure for P43120
Immunogen
HHEX / HEX antibody was raised against recombinant fragment expressed in E Coli corresponding to amino acids 100-270 of human Hex.
Immunogen Description
Recombinant fragment expressed in E Coli corresponding to amino acids 100-270 of human Hex.
Immunogen Type
Recombinant protein
Target Species
Human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-HHEX / HEX antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
Application Notes for anti-HHEX / HEX antibody
IF (1:100)
IHC-P (2.5 ug/ml)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-HHEX / HEX antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-HHEX / HEX Monoclonal Antibody – Cat# MBS249287

Immunofluorescence (IF) of anti-HHEX / HEX antibody

Anti-HHex mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HHex.
Mouse Anti-HHEX / HEX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249287

Immunofluorescence (IF) of anti-HHEX / HEX antibody

Immunofluorescent staining of HT29 cells using anti-HHex mouse monoclonal antibody.
Mouse Anti-HHEX / HEX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249287

Immunofluorescence (IF) of anti-HHEX / HEX antibody

Immunofluorescent staining of HeLa cells using anti-HHex mouse monoclonal antibody.
Mouse Anti-HHEX / HEX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249287

Other Notes

Small volumes of anti-HHEX / HEX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HHEX / HEX purchase

MBS249287 is an antibody targeting HHEX / HEX. Also known as Anti-HHEX / HEX Antibody (clone 3F4) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HHEX / HEX. It may not necessarily be applicable to this product.

NCBI GI #
1170322
NCBI GeneID
15242
NCBI Accession #
P43120.1
UniProt Primary Accession #
P43120
UniProt Secondary Accession #
Q544N6; Q9CRV1; Q9R1X2
UniProt Related Accession #
P43120
NCBI Official Full Name
Hematopoietically-expressed homeobox protein Hhex
NCBI Official Synonym Full Names
hematopoietically expressed homeobox
NCBI Official Symbol
Hhex
NCBI Official Synonym Symbols
Hex; Prh; Hex1; Prhx; Hhex-rs2
UniProt Protein Name
Hematopoietically-expressed homeobox protein Hhex
UniProt Synonym Protein Names
Homeobox protein PRH
UniProt Gene Name
Hhex
UniProt Synonym Gene Names
Prh; Prhx; Homeobox protein HEX; mHex
UniProt Entry Name
HHEX_MOUSE
NCBI Protein Information
hematopoietically-expressed homeobox protein Hhex

UniProt Comments for HHEX / HEX

HHEX: Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

Protein type: Transcription factor; DNA-binding

Cellular Component: cytoplasm; nucleus

Molecular Function: chromatin binding; DNA bending activity; DNA binding; eukaryotic initiation factor 4E binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: anterior/posterior pattern formation; B cell differentiation; cell differentiation; cell proliferation; embryonic heart tube development; embryonic organ development; endoderm development; forebrain development; forebrain morphogenesis; hemopoiesis; in utero embryonic development; interkinetic nuclear migration; liver development; morphogenesis of an epithelium; mRNA export from nucleus; multicellular organism growth; multicellular organismal development; myeloid leukocyte differentiation; negative regulation of angiogenesis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of vascular endothelial growth factor receptor signaling pathway; Notch signaling pathway; organ morphogenesis; pancreas development; poly(A)+ mRNA export from nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; signal transduction; thyroid gland development; tissue morphogenesis; transcription, DNA-dependent; vasculogenesis; Wnt receptor signaling pathway
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Research Articles on HHEX / HEX

1. Data suggest that Hhex is a novel regulator of c-Myc function that limits c-Myc activity in transformed cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HHEX / HEX antibody

 Reference Product  PubMed Publications
 Cdkal1 antibody  >97 publications with HHEX / HEX and Cdkal1
 Slc30a8 antibody  >87 publications with HHEX / HEX and Slc30a8
 Igf2bp2 antibody  >74 publications with HHEX / HEX and Igf2bp2
 Foxa2 antibody  >7 publications with HHEX / HEX and Foxa2
 Gata2 antibody  >2 publications with HHEX / HEX and Gata2
 Eomes antibody  >1 publications with HHEX / HEX and Eomes
 Kdr antibody  >1 publications with HHEX / HEX and Kdr

Pathways associated with anti-HHEX / HEX antibody

 Products by Pathway  Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-HHEX / HEX antibody

 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >126 publications with HHEX / HEX and Diabetes Mellitus, Type 2
 Kidney Diseases Antibodies  >9 publications with HHEX / HEX and Kidney Diseases
 Brain Diseases Antibodies  >9 publications with HHEX / HEX and Brain Diseases
 Liver Diseases Antibodies  >9 publications with HHEX / HEX and Liver Diseases
 Heart Diseases Antibodies  >8 publications with HHEX / HEX and Heart Diseases
 Vascular Diseases Antibodies  >8 publications with HHEX / HEX and Vascular Diseases
 Liver Neoplasms Antibodies  >7 publications with HHEX / HEX and Liver Neoplasms
 Disease Models, Animal Antibodies  >5 publications with HHEX / HEX and Disease Models, Animal
 Abnormalities, Multiple Antibodies  >5 publications with HHEX / HEX and Abnormalities, Multiple
 Inflammation Antibodies  >4 publications with HHEX / HEX and Inflammation

Organs/Tissues associated with anti-HHEX / HEX antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >58 publications with HHEX / HEX and Liver
 Embryonic Tissue Antibodies  >36 publications with HHEX / HEX and Embryonic Tissue
 Heart Antibodies  >17 publications with HHEX / HEX and Heart
 Bone Marrow Antibodies  >10 publications with HHEX / HEX and Bone Marrow
 Intestine Antibodies  >5 publications with HHEX / HEX and Intestine
 Ovary Antibodies  >3 publications with HHEX / HEX and Ovary
 Mammary Gland Antibodies  >1 publications with HHEX / HEX and Mammary Gland
 Spleen Antibodies  >1 publications with HHEX / HEX and Spleen
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