• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LOX Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249296

LOX Antibody, Lysyl oxidase Antibody, Protein-lysine 6-oxidase Antibody Description: Lysyl Oxidase / LOX is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
LOX / Lysyl Oxidase, Monoclonal Antibody
Full Product Name
Anti-LOX / Lysyl Oxidase Antibody (clone 9A11) IHC-plus
Product Gene Name
anti-LOX antibody
Product Synonym Names
Mouse Monoclonal [clone 9A11] (IgG1) to Human LOX / Lysyl Oxidase; Human LOX / Lysyl Oxidase; Lysyl oxidase; Protein-lysine 6-oxidase
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9A11
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
0.8 mg/ml (lot specific)
Specificity
Human Lysyl Oxidase / LOX
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
46,944 Da
OMIM
153455
3D Structure
ModBase 3D Structure for P28300
Immunogen
LOX / Lysyl Oxidase antibody was raised against human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.
Immunogen Description
Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-LOX antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-LOX antibody
Flow Cytometry (1:100)
IF (1:50 - 1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Immunohistochemistry (IHC) of anti-LOX antibody

Anti-Lysyl Oxidase / LOX antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-LOX Monoclonal Antibody – Cat# MBS249296

Immunofluorescence (IF) of anti-LOX antibody

Immunofluorescent staining of HepG2 cells using anti-LOX mouse monoclonal antibody.
Mouse Anti-LOX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249296

Immunofluorescence (IF) of anti-LOX antibody

Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.
Mouse Anti-LOX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249296

Western Blot (WB) of anti-LOX antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
Mouse Anti-LOX Monoclonal Antibody, Western Blot (WB) – Cat# MBS249296

Flow Cytometry (FC/FACS) of anti-LOX antibody

HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
Mouse Anti-LOX Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249296

Flow Cytometry (FC/FACS) of anti-LOX antibody

Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Mouse Anti-LOX Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249296

Flow Cytometry (FC/FACS) of anti-LOX antibody

Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Mouse Anti-LOX Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249296

Other Notes

Small volumes of anti-LOX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LOX purchase

MBS249296 is an antibody targeting LOX. Also known as Anti-LOX / Lysyl Oxidase Antibody (clone 9A11) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LOX. It may not necessarily be applicable to this product.

NCBI GI #
20149540
NCBI GeneID
4015
NCBI Accession #
NP_002308.2
UniProt Primary Accession #
P28300
UniProt Secondary Accession #
Q5FWF0; B2R5Q3
UniProt Related Accession #
P28300
GenBank Nucleotide #
NM_002317.6
NCBI Official Full Name
protein-lysine 6-oxidase isoform 1 preproprotein
NCBI Official Synonym Full Names
lysyl oxidase
NCBI Official Symbol
LOX
UniProt Protein Name
Protein-lysine 6-oxidase
UniProt Synonym Protein Names
Lysyl oxidase
Protein Family
Linoleate 9/13-lipoxygenase
UniProt Gene Name
LOX
UniProt Entry Name
LYOX_HUMAN
NCBI Protein Information
protein-lysine 6-oxidase

NCBI Summary for LOX

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. [provided by RefSeq, Nov 2015]
Show more

UniProt Comments for LOX

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; Secreted; EC 1.4.3.13; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: collagen; extracellular region; extracellular space; nucleus; proteinaceous extracellular matrix

Molecular Function: copper ion binding; protein binding; protein-lysine 6-oxidase activity

Biological Process: collagen fibril organization; elastic fiber assembly; extracellular matrix organization and biogenesis; heart development; lung development; protein modification process; response to drug; response to steroid hormone stimulus; wound healing

Disease: Cutis Laxa, Autosomal Recessive, Type Ia
Show more

Research Articles on LOX

1. LOX gene expression is a predictive factor in hepatocellular liver cancer prognosis and mortality.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LOX antibody

 Reference Product  PubMed Publications
 LOXL1 antibody  >59 publications with LOX and LOXL1
 SRC antibody  >32 publications with LOX and SRC
 FGF2 antibody  >14 publications with LOX and FGF2
 BMP1 antibody  >8 publications with LOX and BMP1
 FBLN5 antibody  >8 publications with LOX and FBLN5
 SNAI1 antibody  >1 publications with LOX and SNAI1
 EFEMP2 antibody  >1 publications with LOX and EFEMP2

Pathways associated with anti-LOX antibody

 Products by Pathway  Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Crosslinking Of Collagen Fibrils Pathway antibodies  Crosslinking Of Collagen Fibrils Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram

Diseases associated with anti-LOX antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >659 publications with LOX and Inflammation
 Cardiovascular Diseases Antibodies  >621 publications with LOX and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >419 publications with LOX and Disease Models, Animal
 Necrosis Antibodies  >249 publications with LOX and Necrosis
 Heart Diseases Antibodies  >192 publications with LOX and Heart Diseases
 Neoplasms, Experimental Antibodies  >156 publications with LOX and Neoplasms, Experimental
 Hypertension Antibodies  >150 publications with LOX and Hypertension
 Fibrosis Antibodies  >150 publications with LOX and Fibrosis
 Lung Neoplasms Antibodies  >130 publications with LOX and Lung Neoplasms
 Breast Neoplasms Antibodies  >119 publications with LOX and Breast Neoplasms

Organs/Tissues associated with anti-LOX antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >770 publications with LOX and Vascular
 Muscle Antibodies  >422 publications with LOX and Muscle
 Brain Antibodies  >391 publications with LOX and Brain
 Lung Antibodies  >303 publications with LOX and Lung
 Heart Antibodies  >282 publications with LOX and Heart
 Liver Antibodies  >260 publications with LOX and Liver
 Bone Antibodies  >248 publications with LOX and Bone
 Skin Antibodies  >186 publications with LOX and Skin
 Kidney Antibodies  >174 publications with LOX and Kidney
 Embryonic Tissue Antibodies  >162 publications with LOX and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000