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TFPI / LACI Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249299

TFPI Antibody, Anti-convertin Antibody, Extrinsic pathway inhibitor Antibody, EPI Antibody, LACI Antibody, TFI Antibody, TFPI1 Antibody Description: TFPI / LACI is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TFPI / LACI, Monoclonal Antibody
Full Product Name
Anti-TFPI / LACI Antibody (aa29-304, clone 3B1) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 3B1] (IgG1) to Human TFPI / LACI; Human TFPI / LACI; Anti-convertin; Extrinsic pathway inhibitor; EPI; TFI; TFPI1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Immunoaffinity purified
Concentration
1 mg/ml (lot specific)
Specificity
Human TFPI / LACI
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
28,653 Da
OMIM
152310
3D Structure
ModBase 3D Structure for P10646
Immunogen
TFPI / LACI antibody was raised against human recombinant protein fragment corresponding to amino acids 29-304 of human TFPI (NP_006278) produced in E Coli.
Immunogen Description
Human recombinant protein fragment corresponding to amino acids 29-304 of human TFPI (NP_006278) produced in E Coli.
Immunogen Type
Recombinant protein
Antigen Modification
aa29-304
Target Species
Human
Applications Tested/Suitable for anti-TFPI / LACI antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-TFPI / LACI antibody
IHC-P (15 ug/ml)
WB (1:500)

Immunohistochemistry - Paraffin (IHC) of anti-TFPI / LACI antibody

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-TFPI / LACI Monoclonal Antibody – Cat# MBS249299

Western Blot (WB) of anti-TFPI / LACI antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFPI.
Mouse Anti-TFPI / LACI Monoclonal Antibody, Western Blot (WB) – Cat# MBS249299

Other Notes

Small volumes of anti-TFPI / LACI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFPI / LACI purchase

MBS249299 is an antibody targeting TFPI / LACI. Also known as Anti-TFPI / LACI Antibody (aa29-304, clone 3B1) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TFPI / LACI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tissue factor pathway inhibitor isoform a
NCBI Official Synonym Full Names
tissue factor pathway inhibitor
NCBI Official Symbol
TFPI
NCBI Official Synonym Symbols
EPI; TFI; LACI; TFPI1
UniProt Protein Name
Tissue factor pathway inhibitor
UniProt Synonym Protein Names
Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI
UniProt Gene Name
TFPI
UniProt Synonym Gene Names
LACI; TFPI1; TFPI; EPI; LACI
UniProt Entry Name
TFPI1_HUMAN
NCBI Protein Information
tissue factor pathway inhibitor

NCBI Summary for TFPI / LACI

This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
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UniProt Comments for TFPI / LACI

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Membrane protein, GPI anchor; Inhibitor

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: cell surface; endoplasmic reticulum; extracellular region; extracellular space; plasma membrane

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: blood coagulation; blood coagulation, extrinsic pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFPI / LACI antibody

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