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PHF7 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249342

PHF7 Antibody, NYD-SP6 Antibody, HSPC226 Antibody, PHD finger protein 7 Antibody, HSPC045 Antibody Description: Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PHF7, Monoclonal Antibody
Full Product Name
Anti-PHF7 Antibody (clone 1A11) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 1A11] (IgG1) to Human PHF7; Human PHF7; NYD-SP6; HSPC226; PHD finger protein 7; HSPC045
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A11
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
1 mg/ml (lot specific)
Specificity
Human PHF7
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
39,259 Da
3D Structure
ModBase 3D Structure for Q9BWX1
Immunogen
PHF7 antibody was raised against human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7(NP_057567) produced in E Coli.
Immunogen Description
Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7(NP_057567) produced in E Coli.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-PHF7 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PHF7 antibody
Flow Cytometry (1:100)
IF (1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Immunohistochemistry (IHC) of anti-PHF7 antibody

Anti-PHF7 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-PHF7 Monoclonal Antibody – Cat# MBS249342

Immunofluorescence (IF) of anti-PHF7 antibody

Anti-PHF7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PHF7.
Mouse Anti-PHF7 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249342

Western Blot (WB) of anti-PHF7 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHF7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHF7.
Mouse Anti-PHF7 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249342

Flow Cytometry (FC/FACS) of anti-PHF7 antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PHF7 antibody, and then analyzed by flow cytometry.
Mouse Anti-PHF7 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249342

Other Notes

Small volumes of anti-PHF7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHF7 purchase

MBS249342 is an antibody targeting PHF7. Also known as Anti-PHF7 Antibody (clone 1A11) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PHF7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PHD finger protein 7 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 7
NCBI Official Symbol
PHF7
NCBI Official Synonym Symbols
HSPC045; HSPC226; NYD-SP6
UniProt Protein Name
PHD finger protein 7
UniProt Synonym Protein Names
Testis development protein NYD-SP6
Protein Family
UniProt Gene Name
PHF7
UniProt Entry Name
PHF7_HUMAN
NCBI Protein Information
PHD finger protein 7

NCBI Summary for PHF7

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
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UniProt Comments for PHF7

PHF7: May play a role in spermatogenesis.

Protein type: Cell development/differentiation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: microtubule organizing center; nucleoplasm; nucleus

Molecular Function: protein binding; zinc ion binding

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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