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ALPI Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249351

ALPI Antibody, Alkaline phosphomonoesterase Antibody, Kasahara isozyme Antibody, Glycerophosphatase Antibody, Alkaline Phosphatase Antibody, IAP Antibody Description: There are at least four distinct but related alkaline phosphatases: intestinal, placental,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ALPI / Alkaline Phosphatase, Monoclonal Antibody
Full Product Name
Anti-ALPI / Alkaline Phosphatase Antibody (aa397-458, clone 3B10E7) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 3B10E7] (IgG1) to Human ALPI / Alkaline Phosphatase; Human ALPI / Alkaline Phosphatase; Alkaline phosphomonoesterase; Kasahara isozyme; Glycerophosphatase; Alkaline Phosphatase; IAP
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B10E7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, 0.05% sodium azide, 0.5% protein stabilizer
Purity/Purification
Protein G purified
Concentration
1 mg/ml (lot specific)
Specificity
Human ALPI / Alkaline Phosphatase
Preparation and Storage
Short term: 4 degree C
Long term: -20 degree C
Molecular Weight
56,812 Da
OMIM
171740
3D Structure
ModBase 3D Structure for P09923
Immunogen
ALPI / Alkaline Phosphatase antibody was raised against purified recombinant fragment of human ALPI (AA: 397-458)
Immunogen Description
Purified recombinant fragment of human ALPI (AA: 397-458)
Immunogen Type
Recombinant protein
Antigen Modification
aa397-458
Target Species
Human
Applications Tested/Suitable for anti-ALPI antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-ALPI antibody
ELISA (1:10000)
Flow Cytometry (1:200 - 1:400)
IHC-P (10 ug/ml)
WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-ALPI antibody

Anti-ALPI / Alkaline Phosphatase antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-ALPI Monoclonal Antibody – Cat# MBS249351

Immunohistochemistry (IHC) of anti-ALPI antibody

Anti-ALPI / Alkaline Phosphatase antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-ALPI Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249351

Western Blot (WB) of anti-ALPI antibody

Western blot using ALPI monoclonal antibody against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
Mouse Anti-ALPI Monoclonal Antibody, Western Blot (WB) – Cat# MBS249351

Western Blot (WB) of anti-ALPI antibody

Western blot using ALPI monoclonal antibody against human ALPI recombinant protein. (Expected MW is 31.9 kDa)
Mouse Anti-ALPI Monoclonal Antibody, Western Blot (WB) – Cat# MBS249351

Western Blot (WB) of anti-ALPI antibody

Western blot using ALPI mouse monoclonal antibody against HL60 (1) and HepG2 (2) cell lysate.
Mouse Anti-ALPI Monoclonal Antibody, Western Blot (WB) – Cat# MBS249351

ELISA (EIA) of anti-ALPI antibody

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Mouse Anti-ALPI Monoclonal Antibody, ELISA (EIA) – Cat# MBS249351

Other Notes

Small volumes of anti-ALPI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALPI purchase

MBS249351 is an antibody targeting ALPI. Also known as Anti-ALPI / Alkaline Phosphatase Antibody (aa397-458, clone 3B10E7) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ALPI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
intestinal-type alkaline phosphatase
NCBI Official Synonym Full Names
alkaline phosphatase, intestinal
NCBI Official Symbol
ALPI
NCBI Official Synonym Symbols
IAP
UniProt Protein Name
Intestinal-type alkaline phosphatase
UniProt Gene Name
ALPI
UniProt Synonym Gene Names
IAP; Intestinal alkaline phosphatase
UniProt Entry Name
PPBI_HUMAN
NCBI Protein Information
intestinal-type alkaline phosphatase

NCBI Summary for ALPI

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]
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UniProt Comments for ALPI

ALPI: intestinal alkaline phosphatase is one of four related alkaline phosphatases. The exact physiological function of alkaline phosphatases is not known. This protein is a GPI-anchored membrane bound glycosylated enzyme that is localized to fetal and adult intestines.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; EC 3.1.3.1; Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Phosphatase

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: alkaline phosphatase activity; magnesium ion binding; protease binding; protein binding; zinc ion binding

Biological Process: dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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