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SAMHD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249374

SAMHD1 Antibody, AGS5 Antibody, CHBL2 Antibody, DCIP Antibody, HDDC1 Antibody, Monocyte protein 5 Antibody, MOP-5 Antibody, MOP5 Antibody, SBBI88 Antibody, Mg11 Antibody, SAM domain and HD domain 1 Antibody Description: Host restriction nuclease that blocks… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SAMHD1, Monoclonal Antibody
Full Product Name
Anti-SAMHD1 Antibody (clone 3F5) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 3F5] (IgG2b) to Human SAMHD1; Human SAMHD1; AGS5; CHBL2; DCIP; HDDC1; Monocyte protein 5; MOP-5; MOP5; SBBI88; Mg11; SAM domain and HD domain 1
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F5
Host
Mouse
Species Reactivity
Mouse, Dog, Rat, Human, Monkey
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SAMHD1 / DCIP
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
68,122 Da
OMIM
606754
3D Structure
ModBase 3D Structure for Q9Y3Z3
Immunogen
SAMHD1 antibody was raised against full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-SAMHD1 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-SAMHD1 antibody
Flow Cytometry (1:100)
IF (1:100)
IHC-P (5 ug/ml)
WB (1:200 - 1:500)

Immunohistochemistry (IHC) of anti-SAMHD1 antibody

Anti-SAMHD1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-SAMHD1 Monoclonal Antibody – Cat# MBS249374

Immunofluorescence (IF) of anti-SAMHD1 antibody

Anti-SAMHD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.
Mouse Anti-SAMHD1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249374

Western Blot (WB) of anti-SAMHD1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Mouse Anti-SAMHD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249374

Western Blot (WB) of anti-SAMHD1 antibody

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
Mouse Anti-SAMHD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249374

Flow Cytometry (FC/FACS) of anti-SAMHD1 antibody

Flow cytometry of HeLa cells, using anti-SAMHD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-SAMHD1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249374

Flow Cytometry (FC/FACS) of anti-SAMHD1 antibody

Flow cytometry of Jurkat cells, using anti-SAMHD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-SAMHD1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249374

Other Notes

Small volumes of anti-SAMHD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAMHD1 purchase

MBS249374 is an antibody targeting SAMHD1. Also known as Anti-SAMHD1 Antibody (clone 3F5) IHC-plus. Reactive in Mouse, Dog, Rat, Human, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SAMHD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
deoxynucleoside triphosphate triphosphohydrolase SAMHD1
NCBI Official Synonym Full Names
SAM domain and HD domain 1
NCBI Official Symbol
SAMHD1
NCBI Official Synonym Symbols
DCIP; CHBL2; HDDC1; MOP-5; SBBI88
UniProt Protein Name
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
UniProt Synonym Protein Names
Dendritic cell-derived IFNG-induced protein; DCIP; Monocyte protein 5; MOP-5; SAM domain and HD domain-containing protein 1
UniProt Gene Name
SAMHD1
UniProt Synonym Gene Names
MOP5; dNTPase; DCIP; MOP-5
UniProt Entry Name
SAMH1_HUMAN
NCBI Protein Information
deoxynucleoside triphosphate triphosphohydrolase SAMHD1

NCBI Summary for SAMHD1

This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq, Mar 2010]
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UniProt Comments for SAMHD1

SAMHD1: a protein induced by interferon gamma.

Protein type: EC 3.1.4.-

Chromosomal Location of Human Ortholog: 20pter-q12

Cellular Component: intracellular; nucleoplasm; nucleus; plasma membrane

Molecular Function: dGTP binding; dGTPase activity; nucleic acid binding; protein binding; RNA binding; zinc ion binding

Biological Process: cytokine and chemokine mediated signaling pathway; dATP catabolic process; defense response to virus; dGTP catabolic process; immune response; protein homotetramerization; regulation of innate immune response

Disease: Aicardi-goutieres Syndrome 5; Chilblain Lupus 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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