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DAPK2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249398

DAPK2 Antibody, DAP-kinase-related protein 1 Antibody, DRP-1 Antibody, DRP1 Antibody, DAP kinase 2 Antibody Description: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival,… More details

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Product Overview

Product Name
DAPK2 / DAP Kinase 2, Monoclonal Antibody
Full Product Name
Anti-DAPK2 / DAP Kinase 2 Antibody (clone 1B5) IHC-plus
Product Gene Name
anti-DAPK2 antibody
Product Synonym Names
Mouse Monoclonal [clone 1B5] (IgG2b) to Human DAPK2 / DAP Kinase 2; Human DAPK2 / DAP Kinase 2; DAP-kinase-related protein 1; DRP-1; DRP1; DAP kinase 2
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1B5
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Protein A/G purified
Concentration
1 mg/ml (lot specific)
Specificity
Human DAPK2
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
55,931 Da
OMIM
616567
3D Structure
ModBase 3D Structure for Q9UIK4
Immunogen
DAPK2 / DAP Kinase 2 antibody was raised against full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-DAPK2 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-DAPK2 antibody
IF (1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Immunohistochemistry (IHC) of anti-DAPK2 antibody

Anti-DAPK2 / DRP1 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-DAPK2 Monoclonal Antibody – Cat# MBS249398

Immunofluorescence (IF) of anti-DAPK2 antibody

Anti-DAPK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2.
Mouse Anti-DAPK2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249398

Western Blot (WB) of anti-DAPK2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.
Mouse Anti-DAPK2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249398

Immunoprecipitation (IP) of anti-DAPK2 antibody

Immunoprecipitation(IP) of DAPK2 by using monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-DAPK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Mouse Anti-DAPK2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS249398

Other Notes

Small volumes of anti-DAPK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DAPK2 purchase

MBS249398 is an antibody targeting DAPK2. Also known as Anti-DAPK2 / DAP Kinase 2 Antibody (clone 1B5) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DAPK2. It may not necessarily be applicable to this product.

NCBI GI #
14670383
NCBI GeneID
23604
NCBI Accession #
NP_055141.2
UniProt Primary Accession #
Q9UIK4
UniProt Secondary Accession #
O75892; Q24JS1; E9JGM7
UniProt Related Accession #
Q9UIK4
GenBank Nucleotide #
NM_014326.3
NCBI Official Full Name
death-associated protein kinase 2
NCBI Official Synonym Full Names
death-associated protein kinase 2
NCBI Official Symbol
DAPK2
NCBI Official Synonym Symbols
DRP1; DRP-1
UniProt Protein Name
Death-associated protein kinase 2
UniProt Synonym Protein Names
DAP-kinase-related protein 1; DRP-1
Protein Family
Death-associated protein kinase
UniProt Gene Name
DAPK2
UniProt Synonym Gene Names
DAP kinase 2; DRP-1
UniProt Entry Name
DAPK2_HUMAN
NCBI Protein Information
death-associated protein kinase 2

NCBI Summary for DAPK2

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]
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UniProt Comments for DAPK2

DAPK2: an ubiquitously expressed protein kinase of the DAPK family. Activated by Ca(2+)/calmodulin. Contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain. Overexpression induces apoptosis. Expressed at high levels in heart, lung and skeletal muscle.

Protein type: EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm; cytoplasmic vesicle

Molecular Function: ATP binding; calmodulin binding; identical protein binding; protein binding; protein serine/threonine kinase activity

Biological Process: anoikis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy
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Research Articles on DAPK2

1. This study links adipocyte expression of an autophagy-regulating kinase, lysosome-mediated clearance and fat cell lipid accumulation; it demonstrates obesity-related attenuated autophagy in adipocytes, and identifies DAPK2 dependence in this regulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DAPK2 antibody

 Reference Product  PubMed Publications
 DAP antibody  >149 publications with DAPK2 and DAP
 CAMK2A antibody  >1 publications with DAPK2 and CAMK2A

Pathways associated with anti-DAPK2 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Caspase Activation Via Extrinsic Apoptotic Signalig Pathway antibodies  Caspase Activation Via Extrinsic Apoptotic Signalig Pathway Diagram
 Ligand-independent Caspase Activation Via DCC Pathway antibodies  Ligand-independent Caspase Activation Via DCC Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Programmed Cell Death Pathway antibodies  Programmed Cell Death Pathway Diagram

Diseases associated with anti-DAPK2 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >68 publications with DAPK2 and Brain Diseases
 Necrosis Antibodies  >61 publications with DAPK2 and Necrosis
 Neoplasm Metastasis Antibodies  >42 publications with DAPK2 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >35 publications with DAPK2 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >34 publications with DAPK2 and Breast Neoplasms
 Inflammation Antibodies  >33 publications with DAPK2 and Inflammation
 Disease Models, Animal Antibodies  >26 publications with DAPK2 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >25 publications with DAPK2 and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >15 publications with DAPK2 and Heart Diseases
 Kidney Diseases Antibodies  >12 publications with DAPK2 and Kidney Diseases

Organs/Tissues associated with anti-DAPK2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >107 publications with DAPK2 and Blood
 Brain Antibodies  >80 publications with DAPK2 and Brain
 Lung Antibodies  >75 publications with DAPK2 and Lung
 Vascular Antibodies  >40 publications with DAPK2 and Vascular
 Stomach Antibodies  >32 publications with DAPK2 and Stomach
 Lymph Node Antibodies  >30 publications with DAPK2 and Lymph Node
 Bone Marrow Antibodies  >20 publications with DAPK2 and Bone Marrow
 Prostate Antibodies  >16 publications with DAPK2 and Prostate
 Skin Antibodies  >11 publications with DAPK2 and Skin
 Intestine Antibodies  >9 publications with DAPK2 and Intestine
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