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TUBB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249431

TUBB Antibody, Beta Tubulin Antibody, Beta 5-tubulin Antibody, Beta-4 tubulin Antibody, Beta1-tubulin Antibody, M40 Antibody, Tubulin beta-5 chain Antibody, Beta Ib tubulin Antibody, Class I beta-tubulin Antibody, OK/SW-cl.56 Antibody, TUBB5 Antibody, Tubulin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TUBB / Beta Tubulin, Monoclonal Antibody
Full Product Name
Anti-TUBB / Beta Tubulin Antibody IHC-plus
Product Gene Name
anti-TUBB antibody
Product Synonym Names
Mouse Monoclonal (IgG2b) to Human TUBB / Beta Tubulin; Human TUBB / Beta Tubulin; TUBB; Beta Tubulin; Beta 5-tubulin; Beta-4 tubulin; Beta1-tubulin; M40; Tubulin beta-5 chain; Beta Ib tubulin; Class I beta-tubulin; OK/SW-cl.56; TUBB5; Tubulin beta chain; Tubulin beta polypeptide; Tubulin beta-1 chain; Tubulin; beta class I
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Species Reactivity
Mouse, Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein G purified
Specificity
Human TUBB / Beta Tubulin
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Molecular Weight
49,671 Da
OMIM
191130
3D Structure
ModBase 3D Structure for P07437
Immunogen
TUBB / Beta Tubulin antibody was raised against tBB5 recombinant protein
Immunogen Description
TBB5 recombinant protein
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-TUBB antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-TUBB antibody
IF (1:10 - 1:50)
IHC-P (1:50)
WB (1:100 - 1:500)

Immunohistochemistry (IHC) of anti-TUBB antibody

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-TUBB Monoclonal Antibody – Cat# MBS249431

Immunofluorescence (IF) of anti-TUBB antibody

Immunofluorescence of A549 cells, using TBB5 Antibody. Antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Mouse Anti-TUBB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249431

Western Blot (WB) of anti-TUBB antibody

Western blot of anti-TBB5 Antibody in Y79,CEM,293 cell line and mouse brain tissue lysates (35 ug/lane). TBB5(arrow) was detected using the purified antibody.(1 ug/ml)
Mouse Anti-TUBB Monoclonal Antibody, Western Blot (WB) – Cat# MBS249431

Other Notes

Small volumes of anti-TUBB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBB purchase

MBS249431 is an antibody targeting TUBB. Also known as Anti-TUBB / Beta Tubulin Antibody IHC-plus. Reactive in Mouse, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBB. It may not necessarily be applicable to this product.

NCBI GI #
29788785
NCBI GeneID
203068
NCBI Accession #
NP_821133.1
UniProt Primary Accession #
P07437
UniProt Secondary Accession #
P05218; Q8WUC1; Q9CY33
UniProt Related Accession #
P07437
GenBank Nucleotide #
NM_178014.3
NCBI Official Full Name
tubulin beta chain isoform b
NCBI Official Synonym Full Names
tubulin beta class I
NCBI Official Symbol
TUBB
NCBI Official Synonym Symbols
M40; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56
UniProt Protein Name
Tubulin beta chain
UniProt Synonym Protein Names
Tubulin beta-5 chain
Protein Family
Tubulin
UniProt Gene Name
TUBB
UniProt Synonym Gene Names
TUBB5
UniProt Entry Name
TBB5_HUMAN
NCBI Protein Information
tubulin beta chain

NCBI Summary for TUBB

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
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UniProt Comments for TUBB

TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cytoskeleton; extracellular region; microtubule; nuclear envelope lumen; nucleus; protein complex

Molecular Function: GTP binding; GTPase activating protein binding; GTPase activity; MHC class I protein binding; protein binding; protein complex binding; protein domain specific binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding

Biological Process: cell division; cell motility; cellular process; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; metabolic process; microtubule-based process; mitotic cell cycle; natural killer cell mediated cytotoxicity; organelle organization and biogenesis; spindle assembly

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6
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Research Articles on TUBB

1. Data suggest that, while lacking a stable structure, NFL-TBS.40-63 peptide (a peptide derived from light neurofilament protein) preferentially binds on a specific single site located near C-terminal end of beta-tubulin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUBB antibody

 Reference Product  PubMed Publications
 ACTB antibody  >14 publications with TUBB and ACTB
 ACTG1 antibody  >2 publications with TUBB and ACTG1

Pathways associated with anti-TUBB antibody

 Products by Pathway  Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Loss Of Nlp From Mitotic Centrosomes Pathway antibodies  Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
 Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway antibodies  Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway Diagram

Diseases associated with anti-TUBB antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >12 publications with TUBB and Nervous System Diseases
 Lung Diseases Antibodies  >9 publications with TUBB and Lung Diseases
 Lung Neoplasms Antibodies  >7 publications with TUBB and Lung Neoplasms
 Brain Diseases Antibodies  >7 publications with TUBB and Brain Diseases
 Hematologic Diseases Antibodies  >6 publications with TUBB and Hematologic Diseases
 Adenocarcinoma Antibodies  >5 publications with TUBB and Adenocarcinoma
 Inflammation Antibodies  >4 publications with TUBB and Inflammation
 Fibrosis Antibodies  >4 publications with TUBB and Fibrosis
 Carcinoma, Squamous Cell Antibodies  >4 publications with TUBB and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >4 publications with TUBB and Neoplasms, Experimental

Organs/Tissues associated with anti-TUBB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >21 publications with TUBB and Blood
 Bone Antibodies  >13 publications with TUBB and Bone
 Brain Antibodies  >12 publications with TUBB and Brain
 Lung Antibodies  >10 publications with TUBB and Lung
 Liver Antibodies  >7 publications with TUBB and Liver
 Vascular Antibodies  >6 publications with TUBB and Vascular
 Bone Marrow Antibodies  >5 publications with TUBB and Bone Marrow
 Intestine Antibodies  >5 publications with TUBB and Intestine
 Eye Antibodies  >4 publications with TUBB and Eye
 Heart Antibodies  >4 publications with TUBB and Heart
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