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MAGEA3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249443

MAGEA3 Antibody, Antigen MZ2-D Antibody, Cancer/testis antigen 1.3 Antibody, CT1.3 Antibody, HYPD Antibody, MAGE-3 antigen Antibody, Melanoma-associated antigen 3 Antibody, HIP8 Antibody, MAGE3 Antibody, Melanoma antigen family A, 3 Antibody Description:… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MAGEA3, Monoclonal Antibody
Full Product Name
Anti-MAGEA3 Antibody (clone 1H1) IHC-plus
Product Gene Name
Product Synonym Names
Mouse Monoclonal [clone 1H1] (IgG1) to Human MAGEA3; Human MAGEA3; Antigen MZ2-D; Cancer/testis antigen 1.3; CT1.3; HYPD; MAGE-3 antigen; Melanoma-associated antigen 3; HIP8; MAGE3; Melanoma antigen family A; 3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1H1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Purity/Purification
Immunoaffinity purified
Concentration
1 mg/ml (lot specific)
Specificity
Human MAGEA3
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
34,747 Da
OMIM
300174
3D Structure
ModBase 3D Structure for P43357
Immunogen
MAGEA3 antibody was raised against full length human recombinant protein of human MAGEA3 (NP_005353) produced in HEK293T cell.
Immunogen Description
Full length human recombinant protein of human MAGEA3 (NP_005353) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Target Species
Human
Applications Tested/Suitable for anti-MAGEA3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-MAGEA3 antibody
IHC-P (10 ug/ml)
WB (1:2000)

Immunohistochemistry - Paraffin (IHC) of anti-MAGEA3 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-MAGEA3 Monoclonal Antibody – Cat# MBS249443

Immunohistochemistry - Paraffin (IHC) of anti-MAGEA3 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-MAGEA3 Monoclonal Antibody, Immunohistochemistry - Paraffin (IHC) – Cat# MBS249443

Western Blot (WB) of anti-MAGEA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA3.
Mouse Anti-MAGEA3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS249443

Other Notes

Small volumes of anti-MAGEA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAGEA3 purchase

MBS249443 is an antibody targeting MAGEA3. Also known as Anti-MAGEA3 Antibody (clone 1H1) IHC-plus. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAGEA3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Melanoma-associated antigen 3
NCBI Official Synonym Full Names
MAGE family member A3
NCBI Official Symbol
MAGEA3
NCBI Official Synonym Symbols
HIP8; HYPD; CT1.3; MAGE3; MAGEA6
UniProt Protein Name
Melanoma-associated antigen 3
UniProt Synonym Protein Names
Antigen MZ2-D; Cancer/testis antigen 1.3; CT1.3; MAGE-3 antigen
UniProt Gene Name
MAGEA3
UniProt Synonym Gene Names
MAGE3; CT1.3
UniProt Entry Name
MAGA3_HUMAN
NCBI Protein Information
melanoma-associated antigen 3

NCBI Summary for MAGEA3

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAGEA3

MAGEA3: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: endoplasmic reticulum

Molecular Function: protein binding

Biological Process: negative regulation of caspase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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