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LAMP2 / CD107b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249477

LAMP2 Antibody, CD107b Antibody, CD107b antigen Antibody, LAMPB Antibody, LGP110 Antibody, LAMP-2 Antibody Description: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic… More details

$650 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LAMP2 / CD107b, Monoclonal Antibody
Full Product Name
Anti-LAMP2 / CD107b Antibody IHC-plus
Product Gene Name
anti-LAMP2 / CD107b antibody
Product Synonym Names
Mouse Monoclonal (IgG1,k) to Mouse LAMP2 / CD107b; Mouse LAMP2 / CD107b; LAMP2; CD107b; CD107b antigen; LAMPB; LGP110; LAMP-2
Clonality
Monoclonal
Isotype
IgG1,k
Host
Mouse
Species Reactivity
Mouse, Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein G purified
Specificity
Mouse CD107b / LAMP2
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
OMIM
300257
3D Structure
ModBase 3D Structure for P13473
Immunogen Species
LAMP2 / CD107b antibody was raised against Mouse
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-LAMP2 / CD107b antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-LAMP2 / CD107b antibody
IF (1:10 - 1:50)
IHC-P (1:50)
WB (1:100 - 1:1000)

Immunohistochemistry (IHC) of anti-LAMP2 / CD107b antibody

Anti-LAMP2 / CD107b antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Mouse Anti-LAMP2 / CD107b Monoclonal Antibody – Cat# MBS249477

Immunohistochemistry (IHC) of anti-LAMP2 / CD107b antibody

Anti-LAMP2 / CD107b antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Mouse Anti-LAMP2 / CD107b Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249477

Immunofluorescence (IF) of anti-LAMP2 / CD107b antibody

Confocal immunofluorescence of LAMP2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Mouse Anti-LAMP2 / CD107b Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS249477

Western Blot (WB) of anti-LAMP2 / CD107b antibody

LAMP2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The LAMP2 antibody detected the LAMP2 protein (arrow).
Mouse Anti-LAMP2 / CD107b Monoclonal Antibody, Western Blot (WB) – Cat# MBS249477

Other Notes

Small volumes of anti-LAMP2 / CD107b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LAMP2 / CD107b purchase

MBS249477 is an antibody targeting LAMP2 / CD107b. Also known as Anti-LAMP2 / CD107b Antibody IHC-plus. Reactive in Mouse, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LAMP2 / CD107b. It may not necessarily be applicable to this product.

NCBI GI #
7669503
NCBI GeneID
3920
NCBI Accession #
NP_054701.1
UniProt Primary Accession #
P13473
UniProt Secondary Accession #
Q16641; Q6Q3G8; Q96J30; Q99534; Q9UD93; A8K4X5; D3DTF0
UniProt Related Accession #
P13473
GenBank Nucleotide #
NM_013995.2
NCBI Official Full Name
lysosome-associated membrane glycoprotein 2 isoform B
NCBI Official Synonym Full Names
lysosomal associated membrane protein 2
NCBI Official Symbol
LAMP2
NCBI Official Synonym Symbols
LAMPB; CD107b; LAMP-2; LGP110
UniProt Protein Name
Lysosome-associated membrane glycoprotein 2
UniProt Synonym Protein Names
CD107 antigen-like family member B; CD_antigen: CD107b
UniProt Gene Name
LAMP2
UniProt Synonym Gene Names
LAMP-2; Lysosome-associated membrane protein 2
UniProt Entry Name
LAMP2_HUMAN
NCBI Protein Information
lysosome-associated membrane glycoprotein 2

NCBI Summary for LAMP2 / CD107b

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]
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UniProt Comments for LAMP2 / CD107b

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: extracellular space; integral to membrane; late endosome; late endosome membrane; lysosomal lumen; lysosomal membrane; lysosome; membrane; phagocytic vesicle membrane; plasma membrane; platelet dense granule membrane

Molecular Function: enzyme binding; protein binding; protein domain specific binding

Biological Process: blood coagulation; platelet activation; platelet degranulation; protein import; protein stabilization; regulation of protein stability

Disease: Danon Disease
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Research Articles on LAMP2 / CD107b

1. Down-regulation of LAMP2A expression could inhibit cell proliferation in multiple myeloma cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LAMP2 / CD107b antibody

 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram

Diseases associated with anti-LAMP2 / CD107b antibody

 Disease Name  Pubmed Publications
 Neurobehavioral Manifestations Antibodies  >53 publications with LAMP2 / CD107b and Neurobehavioral Manifestations
 Glycogen Storage Disease Type IIb Antibodies  >52 publications with LAMP2 / CD107b and Glycogen Storage Disease Type IIb
 Hypertrophy Antibodies  >27 publications with LAMP2 / CD107b and Hypertrophy
 Immune System Diseases Antibodies  >17 publications with LAMP2 / CD107b and Immune System Diseases
 Inflammation Antibodies  >16 publications with LAMP2 / CD107b and Inflammation
 Movement Disorders Antibodies  >14 publications with LAMP2 / CD107b and Movement Disorders
 Lung Diseases Antibodies  >10 publications with LAMP2 / CD107b and Lung Diseases
 Liver Diseases Antibodies  >9 publications with LAMP2 / CD107b and Liver Diseases
 Necrosis Antibodies  >9 publications with LAMP2 / CD107b and Necrosis
 Fibrosis Antibodies  >6 publications with LAMP2 / CD107b and Fibrosis
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