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C4BPA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249505

C4BPA Antibody, C4BP Antibody, PrP Antibody, Proline-rich protein Antibody Description: C4BPA / C4BP Alpha is a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with… More details

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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
C4BPA / C4BP Alpha, Polyclonal Antibody
Full Product Name
Anti-C4BPA / C4BP Alpha Antibody (aa470-499) IHC-plus
Product Gene Name
anti-C4BPA antibody
Product Synonym Names
Rabbit Polyclonal to Human C4BPA / C4BP Alpha; Human C4BPA / C4BP Alpha; C4BP; PrP; Proline-rich protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein A purified.
Specificity
This C4BPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from the C-terminal region of human C4BPA.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 6 months.
For long term storage store at -20°C.
OMIM
120830
3D Structure
ModBase 3D Structure for P04003
Immunogen
C4BPA / C4BP Alpha antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human C4BPA.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human C4BPA.
Immunogen Type
Synthetic peptide
Target
C4BPA/C4BP Alpha
Epitope
aa470-499
Immunogen Species
Raised against human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-C4BPA antibody
Immunohistochemistry (IHC-P) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-C4BPA antibody
Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)
Optimal dilutions/concentrations should be determined by the end user.

Immunohistochemistry (IHC) of anti-C4BPA antibody

Anti-C4BPA / C4BP antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-C4BPA Polyclonal Antibody – Cat# MBS249505

Western Blot (WB) of anti-C4BPA antibody

C4BPA Antibody western blot of WiDr cell line lysates (35 ug/lane). The C4BPA antibody detected the C4BPA protein (arrow).
Rabbit Anti-C4BPA Polyclonal Antibody, Western Blot (WB) – Cat# MBS249505

Flow Cytometry (FC/FACS) of anti-C4BPA antibody

C4BPA Antibody flow cytometry of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-C4BPA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249505

Other Notes

Small volumes of anti-C4BPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C4BPA purchase

MBS249505 is an antibody targeting C4BPA. Also known as Anti-C4BPA / C4BP Alpha Antibody (aa470-499) IHC-plus. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for C4BPA. It may not necessarily be applicable to this product.

NCBI GI #
4502503
NCBI GeneID
722
NCBI Accession #
NP_000706.1
UniProt Primary Accession #
P04003
UniProt Secondary Accession #
Q5VVQ8
UniProt Related Accession #
P04003
GenBank Nucleotide #
NM_000715.3
NCBI Official Full Name
C4b-binding protein alpha chain
NCBI Official Synonym Full Names
complement component 4 binding protein, alpha
NCBI Official Symbol
C4BPA
NCBI Official Synonym Symbols
PRP; C4BP
UniProt Protein Name
C4b-binding protein alpha chain
UniProt Synonym Protein Names
Proline-rich protein; PRP
Protein Family
C4b-binding protein
UniProt Gene Name
C4BPA
UniProt Synonym Gene Names
C4BP; C4bp; PRP
UniProt Entry Name
C4BPA_HUMAN
NCBI Protein Information
C4b-binding protein alpha chain

NCBI Summary for C4BPA

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]
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UniProt Comments for C4BPA

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: protein binding

Biological Process: complement activation, classical pathway; innate immune response; negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation
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Research Articles on C4BPA

1. whereas the presence of plasminogen did not affect the factor I cofactor activity of C4BP, the activation of plasminogen by urokinase-type plasminogen activator to active plasmin was significantly augmented in the presence of C4BP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-C4BPA antibody

 Reference Product  PubMed Publications
 C4B antibody  >82 publications with C4BPA and C4B
 C4BPB antibody  >23 publications with C4BPA and C4BPB
 CFI antibody  >3 publications with C4BPA and CFI
 APCS antibody  >2 publications with C4BPA and APCS
 PROS1 antibody  >2 publications with C4BPA and PROS1
 C4A antibody  >1 publications with C4BPA and C4A
 CD40 antibody  >1 publications with C4BPA and CD40

Pathways associated with anti-C4BPA antibody

 Products by Pathway  Pathway Diagram
 CD40/CD40L Signaling Pathway antibodies  CD40/CD40L Signaling Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Regulation Of Complement Cascade Pathway antibodies  Regulation Of Complement Cascade Pathway Diagram

Diseases associated with anti-C4BPA antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with C4BPA and Inflammation
 Immune System Diseases Antibodies  >7 publications with C4BPA and Immune System Diseases
 Cardiovascular Diseases Antibodies  >5 publications with C4BPA and Cardiovascular Diseases
 Blood Coagulation Disorders Antibodies  >4 publications with C4BPA and Blood Coagulation Disorders
 Carcinoma, Hepatocellular Antibodies  >4 publications with C4BPA and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >4 publications with C4BPA and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with C4BPA and Liver Neoplasms
 Necrosis Antibodies  >3 publications with C4BPA and Necrosis
 Lung Neoplasms Antibodies  >2 publications with C4BPA and Lung Neoplasms
 Acute Kidney Injury Antibodies  >2 publications with C4BPA and Acute Kidney Injury

Organs/Tissues associated with anti-C4BPA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >41 publications with C4BPA and Blood
 Liver Antibodies  >7 publications with C4BPA and Liver
 Uterus Antibodies  >4 publications with C4BPA and Uterus
 Lung Antibodies  >4 publications with C4BPA and Lung
 Connective Tissue Antibodies  >2 publications with C4BPA and Connective Tissue
 Muscle Antibodies  >2 publications with C4BPA and Muscle
 Brain Antibodies  >2 publications with C4BPA and Brain
 Intestine Antibodies  >1 publications with C4BPA and Intestine
 Mammary Gland Antibodies  >1 publications with C4BPA and Mammary Gland
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