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ZC3H12A / MCPIP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249619

ZC3H12A Antibody, DJ423B22.1 Antibody, MCPIP Antibody, Ribonuclease ZC3H12A Antibody, MCP induced protein 1 Antibody, MCP-induced protein 1 Antibody, MCPIP1 Antibody, RP3-423B22.1 Antibody Description: Has RNase activity and selectively degrades specific… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZC3H12A / MCPIP1, Polyclonal Antibody
Full Product Name
Anti-ZC3H12A / MCPIP1 Antibody (aa331-358) IHC-plus
Product Gene Name
anti-ZC3H12A / MCPIP1 antibody
Product Synonym Names
Rabbit Polyclonal to Human ZC3H12A / MCPIP1; Human ZC3H12A / MCPIP1; ZC3H12A; DJ423B22.1; MCPIP; Ribonuclease ZC3H12A; MCP induced protein 1; MCP-induced protein 1; MCPIP1; RP3-423B22.1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein A purified
Specificity
This ZC12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the Central region of human ZC12A.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
OMIM
610562
3D Structure
ModBase 3D Structure for Q5D1E8
Immunogen Species
ZC3H12A/MCPIP1 antibody was raised against Human
Antigen Modification
aa331-358
Target Species
Human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-ZC3H12A / MCPIP1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ZC3H12A / MCPIP1 antibody
Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)

Optimal dilutions to be determined by the researcher.

Immunohistochemistry (IHC) of anti-ZC3H12A / MCPIP1 antibody

Anti-ZC3H12A / MCPIP1 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-ZC3H12A / MCPIP1 Polyclonal Antibody – Cat# MBS249619

Western Blot (WB) of anti-ZC3H12A / MCPIP1 antibody

ZC12A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The ZC12A antibody detected the ZC12A protein (arrow).
Rabbit Anti-ZC3H12A / MCPIP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS249619

Flow Cytometry (FC/FACS) of anti-ZC3H12A / MCPIP1 antibody

ZC12A Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ZC3H12A / MCPIP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249619

Other Notes

Small volumes of anti-ZC3H12A / MCPIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZC3H12A / MCPIP1 purchase

MBS249619 is an antibody targeting ZC3H12A / MCPIP1. Also known as Anti-ZC3H12A / MCPIP1 Antibody (aa331-358) IHC-plus. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ZC3H12A / MCPIP1. It may not necessarily be applicable to this product.

NCBI GI #
156151383
NCBI GeneID
80149
NCBI Accession #
NP_079355.2
UniProt Primary Accession #
Q5D1E8
UniProt Secondary Accession #
Q6I9Z1; Q9H5P1; D3DPT0
UniProt Related Accession #
Q5D1E8
GenBank Nucleotide #
NM_025079.2
NCBI Official Full Name
ribonuclease ZC3H12A
NCBI Official Synonym Full Names
zinc finger CCCH-type containing 12A
NCBI Official Symbol
ZC3H12A
NCBI Official Synonym Symbols
MCPIP; MCPIP1; dJ423B22.1
UniProt Protein Name
Ribonuclease ZC3H12A
UniProt Synonym Protein Names
MCP-induced protein 1; Zinc finger CCCH domain-containing protein 12A
UniProt Gene Name
ZC3H12A
UniProt Entry Name
ZC12A_HUMAN
NCBI Protein Information
ribonuclease ZC3H12A

NCBI Summary for ZC3H12A / MCPIP1

ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]

UniProt Comments for ZC3H12A / MCPIP1

MCPIP1: Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA. Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19. Belongs to the ZC3H12 family.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: cytoplasm; cytoskeleton; nucleoplasm; nucleus; plasma membrane; rough endoplasmic reticulum

Molecular Function: chromatin binding; DNA binding; endoribonuclease activity; exoribonuclease activity; metal ion binding; miRNA binding; mRNA 3'-UTR binding; mRNA binding; protein binding; ribonuclease activity; RNA binding

Biological Process: angiogenesis; apoptosis; cell differentiation; inhibition of NF-kappaB transcription factor; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta secretion; negative regulation of interleukin-6 production; negative regulation of macrophage activation; negative regulation of NF-kappaB import into nucleus; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of defense response to virus by host; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; protein oligomerization
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Research Articles on ZC3H12A / MCPIP1

1. In this review we summarize current progress regarding the specific characteristics of sequences and structures in the 3' untranslated regions of mRNAs that are recognized by tristetraproline, Roquins, and Regnase-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ZC3H12A / MCPIP1 antibody

 Reference Product  PubMed Publications
 CCL2 antibody  >11 publications with ZC3H12A / MCPIP1 and CCL2
 BECN1 antibody  >2 publications with ZC3H12A / MCPIP1 and BECN1
 FOS antibody  >1 publications with ZC3H12A / MCPIP1 and FOS
 IL1B antibody  >1 publications with ZC3H12A / MCPIP1 and IL1B
 IL2 antibody  >1 publications with ZC3H12A / MCPIP1 and IL2

Diseases associated with anti-ZC3H12A / MCPIP1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >32 publications with ZC3H12A / MCPIP1 and Inflammation
 Necrosis Antibodies  >14 publications with ZC3H12A / MCPIP1 and Necrosis
 Cardiovascular Diseases Antibodies  >11 publications with ZC3H12A / MCPIP1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >9 publications with ZC3H12A / MCPIP1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >7 publications with ZC3H12A / MCPIP1 and Nervous System Diseases
 Lung Diseases Antibodies  >5 publications with ZC3H12A / MCPIP1 and Lung Diseases
 Liver Diseases Antibodies  >5 publications with ZC3H12A / MCPIP1 and Liver Diseases
 Anemia Antibodies  >3 publications with ZC3H12A / MCPIP1 and Anemia
 Breast Neoplasms Antibodies  >2 publications with ZC3H12A / MCPIP1 and Breast Neoplasms
 Carcinoma Antibodies  >2 publications with ZC3H12A / MCPIP1 and Carcinoma

Organs/Tissues associated with anti-ZC3H12A / MCPIP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >7 publications with ZC3H12A / MCPIP1 and Vascular
 Liver Antibodies  >6 publications with ZC3H12A / MCPIP1 and Liver
 Lung Antibodies  >6 publications with ZC3H12A / MCPIP1 and Lung
 Spleen Antibodies  >5 publications with ZC3H12A / MCPIP1 and Spleen
 Brain Antibodies  >4 publications with ZC3H12A / MCPIP1 and Brain
 Muscle Antibodies  >4 publications with ZC3H12A / MCPIP1 and Muscle
 Heart Antibodies  >4 publications with ZC3H12A / MCPIP1 and Heart
 Thymus Antibodies  >2 publications with ZC3H12A / MCPIP1 and Thymus
 Lymph Node Antibodies  >2 publications with ZC3H12A / MCPIP1 and Lymph Node
 Placenta Antibodies  >1 publications with ZC3H12A / MCPIP1 and Placenta
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