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ENO2 / NSE Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249623

ENO2 Antibody, Enolase 2 Antibody, Enolase 2 (gamma, neuronal) Antibody, Neurone-specific enolase Antibody, Gamma-enolase Antibody, Neural enolase Antibody, Neuron specific gamma enolase Antibody, Neuron-specific enolase Antibody, NSE Antibody Description:… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ENO2 / NSE, Polyclonal Antibody
Full Product Name
Anti-ENO2 / NSE Antibody IHC-plus
Product Gene Name
Product Synonym Names
Chicken Polyclonal (IgY); ENO2; Enolase 2; Enolase 2 (gamma; neuronal); Neurone-specific enolase; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Rat, Human
Form/Format
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Purity/Purification
Affinity purified
Specificity
These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 12 months. Sensitive to light.
Molecular Weight
42,707 Da
OMIM
131360
3D Structure
ModBase 3D Structure for P09104
Immunogen
ENO2 / NSE antibody was raised against two synthetic peptide / keyhole limpet hemocyanin
Immunogen Description
Two synthetic peptide / keyhole limpet hemocyanin
Immunogen Type
Synthetic peptide
Applications Tested/Suitable for anti-ENO2 / NSE antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ENO2 / NSE antibody
ICC (1:2000 - 1:5000)
IHC-P (1:500)
WB (1:5000 - 1:10000)

Immunohistochemistry (IHC) of anti-ENO2 / NSE antibody

Anti-ENO2 / NSE antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Chicken Anti-ENO2 / NSE Polyclonal Antibody – Cat# MBS249623

Other Notes

Small volumes of anti-ENO2 / NSE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO2 / NSE purchase

MBS249623 is an antibody targeting ENO2 / NSE. Also known as Anti-ENO2 / NSE Antibody IHC-plus. Reactive in Rat, Human. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO2 / NSE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN
NCBI Protein Information
gamma-enolase

NCBI Summary for ENO2 / NSE

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

UniProt Comments for ENO2 / NSE

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: EC 4.2.1.11; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane

Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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